摘要
目的观察诱导型一氧化氮合酶抑制剂SMT对大鼠门脉高压症食管静脉曲张的影响。方法健康雄性SD大鼠60只随机分为5组,假手术组、模型组、低剂量组、中剂量组和高剂量组。假手术组仅分离门静脉、左肾上腺静脉关腹,其余组门脉缩窄两步法加左肾上腺静脉结扎,建立门脉高压症食管静脉曲张模型。假手术组与模型组手术后给予腹腔注射生理盐水,其余3组手术后给予腹腔注射不同浓度SMT。手术后21 d,检测大鼠门脉血中TNOS、iNOS的活性及NO的浓度,免疫组化CD34标记食管血管内皮,测量每组大鼠食管横切面黏膜下血管的数目、面积。结果模型组大鼠门脉血中TNOS活性与iNOS活性以及NO浓度和食管黏膜下血管数目,血管平均截面积,血管总面积均较假手术组显著升高(P<0.01)。中、高剂量组大鼠门脉血中TNOS活性与iNOS活性以及NO浓度和食管黏膜下血管的数目、血管平均面积、血管总面积较模型组均显著下调(P≤0.01)。结论大鼠门脉高压食管静脉曲张的发病机制中有NO参与,门脉缩窄型门脉高压食管静脉曲张病中NO主要由iNOS生成,SMT对大鼠门脉高压食管静脉曲张可能具有一定保护作用。
Objective To observe the effect of inducible nitric oxide synthase inhibitor SMT on portal hypertension esophageal varices in rats.Methods／ 60 health male SD rats were randomly divided into five groups,sham operation group,model group,low dose SMT treatment group,middle dose SMT treatment group and high dose SMT treatment group.The portal vein and the left adrenal vein were not ligated in sham-operated group.The other groups were given a two-stage ligation of the portal vein plus ligation of the left adrenal vein.Sham-operation group and model group were given intraperitoneal injection of normal saline.Other three groups were given intraperitoneal injection with different concentrations SMT.On 21 days after surgery,portal vein pressure was measured;The total nitric oxide synthase（TNOS） activity,inducible nitric oxide synthase（iNOS） activity and NO content in portal vein were determind;The numbers and area of esophageal submucosal vessels which were marked by CD34 were measured by image analytical apparatus.Results The model group had a significant increase in the TNOS activity,the iNOS activity,the NO content of portal blood,as well as in the number,the average cross-sectional area,the total cross-sectional area of esophageal submucosal vessels comparied with the sham-operation group（P0.01）.Whereas the middle dose SMT treatment group and high dose SMT treatment group have a significant decrease in the above parameters comparied with the model group（P≤0.01）.Conclusions／ NO plays an important role in the pathogenesis of esophageal varices.NO is mainly generated by iNOS in the portal hypertension esophageal varices induced by ligating partially portal vein.SMT have some protective effect on esophageal varices in rats with portal hypertension.
出处
《中国比较医学杂志》
CAS
2010年第3期39-42,84,共5页
Chinese Journal of Comparative Medicine
