摘要
目的:探讨中药寒热药性与其所含蛋白质、总氨基酸、脂类、总糖、多糖及单糖等初生物质的相关性。方法:蛋白质含量测定采用考马斯亮蓝法,总氨基酸含量测定采用柱前衍生液相色谱法,总脂肪测定采用索氏提取法,总糖含量测定采用滴定法,总多糖含量测定采用经典蒽酮比色法,单糖测定按照《中华人民共和国国家标准GB/T5009.7》进行。用统计描述和Fisher判别法进行分析。结果:Fisher判别法分析20种中药寒热药性与其初生物质的相关性,判别正确率达85%。结论:20种中药寒热药性与其所含初生物质具有一定的相关性。Fisher判别法为进一步分析研究中药药性物质基础提供了新的思路和方法。
Objective：To explore the correlation between cold or heat property of 20 kinds of traditional Chinese drugs and the primary materials,which contained proteins,total amino acids,lipids,total sugars,polysaecharides, nmnosaccharides,etc.. Methods：The content of protein in every crude drug was determined by Coomassie Brilliant Blue method;the content of total amino acid was determined with pre-column derivatization by reversed phase high performance liquid chromatography;Soxhlet extraction for determination the quantity of lipids in samples;total sugars was determined by titrimetry;testing total polysaeeharides by Anthrone Colorimetry;testing monosaccharides according to notional standards of the People＇s Republic of China GB/T5OO9.Z The data were analyzed by statistical descrip- tion and Fisher diseriminant methods. Results：The correct rate was up to eighty-five percent about the relation be- tween cold or heat properties of traditional Chinese drugs and the primary materials analyzed by Fisher discriminant methods. Conclusion：There were correlation between properties of twenty traditional Chinese medicines and their pri- mary metabolites. Fisher diseriminant method was feasible to study and analyze the physical basis of properties of traditional Chinese drugs.
出处
《山东中医药大学学报》
2010年第2期99-102,共4页
Journal of Shandong University of Traditional Chinese Medicine
基金
国家"973"计划项目(编号:2007CB512600)
山东省科技发展计划课题(编号:2006GG2202017
2006GG3202003)
关键词
中药
初生物质
含量
药性
traditional Chinese drugs
primary materials
content
property
