摘要
利用变色圈法和噬油斑法从土壤样品中分离筛选出一株产生物表面活性剂的细菌菌株LB345,通过薄层层析(TLC)确定其所产生物表面活性剂为脂肽,并通过单因素实验及L9(34)正交实验对菌株的培养基配方进行了优化,确定优化培养基的组成(g/L):葡萄糖30,NaNO32,KH2PO410,Na2HPO410,FeSO42.5×10-4,MgSO4.7H2O 0.03,NaC l 5,CaC l20.4,酵母粉0.3,初始pH值5,培养温度37℃,发酵时间7 d。在上述条件下,菌株LB345的脂肽产量可达到0.7 g/L。
A biosurfactant producing bacteria strain LB345 was seperated and screened from soil and mud through color zone and oil - rejection zone methods. The results of TLC analysis showed that biosnrfactant of one strain bacteria was lipopeptide . And by means of single factor and L9 （34） ortbogonal tests, the medium formula was optimized（g/L） , dextrose 30, NAN03 2, KH2PO4 10, Na2 HPO4 10, FeSO4 2.5 × 10-4, MgSO4· 7H2O 0.03, NaCl 5, CaC12 0.4, yeast powder 0.3, the initial pH value of medium 5, temperature of medium 37 ℃ and culture duration 7 d. , under above conditions, lipopeptide biosurfactant yield of LB345 was 0.7 g/L.
出处
《辽宁化工》
CAS
2010年第3期236-239,254,共5页
Liaoning Chemical Industry
关键词
脂肽
生物表面活性剂
菌株筛选
培养基优化
Lipopeptide
Biosurfactant
Screening of bacteria
Optimization of medium
