摘要
以涂布法和富集培养法筛选得到降解菌株HQ-C-01,该菌株在48 h时对50 mg/L克百威、甲萘威、茚虫威和仲丁威的降解率分别可达95.2%、99.0%、85.0%和67.5%.确定菌株HQ-C-01的C、N、P源化合物为葡萄糖、蛋白胨和磷酸氢二钾,采用中心组分设计法确定了菌株HQ-C-01的培养基最适C、N、P源配比为葡萄糖32.10 g/L、蛋白胨3.25 g/L、磷酸氢二钾1.50 g/L;在最佳培养基条件下,D590 nm实测值为0.786 5,与期望值0.805 4接近,48 h时对50 mg/L克百威降解率为95.9%.
A high effective stain named HQ-C-01 carbofuran, carbaryl, indoxacarb and fenobucarb was screened, which the degrading rates for 50 mg/L after treatment in 48 h were 95.2%, 99. 0%, 85.0% and 67.5% respectively. It was found that the compounds for C, N and P were glucose, peptone and potassium phosphate dibasic. By using the central composite rotary design method, the results indicated that the optimum of C ,N and P were 32. 10 g/l. for glucose ,3.25 g/L for peptone and 1. 50 g/L for potassium phosphate dibasic respectively. The measured value of D590 was 0. 786 5 similarly to the expected value, and the degrading rate of 50 mg/L carbofuran was 95.9% after 48 h by the strain incubation in medium optimization.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2009年第3期27-31,共5页
Journal of South China Agricultural University
基金
国家自然科学基金(30871660)
关键词
克百威
高效降解菌
筛选
培养基优化
carbofuran
high degrading ability strain
screening
medium optimization
