摘要
目的探讨急性吗啡暴露对大鼠前额叶皮质τ蛋白磷酸化及学习记忆的影响。方法40只雄性SD大鼠随机均分成急性吗啡暴露组(M组)和对照组(C组)。M组单次腹腔注射吗啡(30 mg/kg),C组同法注射等量生理盐水。注射后2 h和24 h分别断头处死10只大鼠,免疫印迹技术检测前额叶皮质τ蛋白的磷酸化水平。后处死的大鼠于注射后2、4及24 h分别行水迷宫实验。结果M组注射后2 h时平台寻找时间显著长于C组(P<0.01)。M组大鼠前额叶皮质τ蛋白Ser396和Thr231位点的磷酸化水平于注射后2 h时显著高于C组(P<0.05)。结论急性吗啡暴露可致大鼠学习记忆功能可逆性减退,前额叶皮质τ蛋白过度磷酸化可能与其有关。
Objective To explore the effect of acute morphine on memory and τ phosphorylation of the prefrontal cortex of rats.Methods Forty SD rats were randomly divided into two groups with 20 rats each.Group M received intraperitoneally injection of morphine 30 mg/kg.Group C was given normal saline instead of morphine as the control.Ten rats from each group were sacrificed to get the prefrontal cortex for detecting τ protein phosphorylation at the sites Ser396 and Thr231 with immunoblotting at 2 h and 24 h after injections.Laterly sacrificed rats in each group were tested with morris water maze（MWM） at 2 h,4 h and 24 h after injections.Results The latent period was significantly longer in group M than that of group C（P〈0.01） at 2 h after injections.The hyperphosphorylation at the sites of Ser396 and Thr231 of τ in group M was significantly higher than that in group C（P〈0.05） at 2 h after injection.Conclusion The intraperitoneally injection of morphine 30 mg/ kg may lead to transient memory decrease in rats,which may be related to the hyperphosphorylation at the sites of Ser396 and Thr231of τ in the prefrontal cortex.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2010年第1期61-63,共3页
Journal of Clinical Anesthesiology
基金
广东省自然科学基金资助项目(No.7001595)
关键词
Τ蛋白
吗啡
磷酸化
τ Proteins
Morphine
Phosphorylation
