摘要
目的探讨兴奋性氨基酸载体1（EAAC1）在吗啡耐受形成机制中的作用。方法将30只雄性SD大鼠行鞘内置管,随机均分为六组。其中的四组于左后肢制成佐剂性关节炎模型,分别经鞘内给予生理盐水（C组）、吗啡10μg（M10组）、吗啡20μg（M20组）、吗啡10μg＋纳洛酮10μg（MN组）;另外两组未致炎大鼠分别经鞘内给予生理盐水（C0组）、吗啡20μg（M0组）。各组给药均为1日2次,连续7 d。动态检测大鼠左后肢50%缩爪阈值,于给药后第8天测定痛阈后将大鼠处死,取左侧L3-4、L4-5节段背根神经节（DRG）进行免疫组织化学染色,检测DRG中EAAC1的表达。结果M10、M20两组在鞘内给予吗啡第8天形成较稳定的吗啡耐受,其DRG内EAAC1表达下调。结论DRG EAAC1参与炎性痛大鼠慢性吗啡耐受的形成机制。
Objective To explore the role of excitatory amino acid carrier 1（EAAC1）in dorsal root ganglion（DRG） in the mechanism of developing morphine tolerance.Methods Thirty male SD rats were implanted intrathecal catheters and randomized into 6 groups with 5 rats each.The rats of 4 groups were made into the model of adjuvant-induced arthritis in the left hind limb and were administered intrathecally,morphine 10 μg（group M10）,morphine 20 μg（group M20）,morphine 20 μg plus naloxone 10 μg（group MN）,or saline（group C） respectively.The other 2 groups without were administered intrathecally saline（group C0） or morphine 20 μg（group M0）.The drugs were administered twice daily for 7 days.Mechanical withdrawl threshold（MWT） of the left hind limb was examined to evaluate the behavior.Immunohistochemistry was used to detect the expression of EAAC1 in the left L3-4 and L4-5 DRG.Results Morphine tolerance was formmed stably in the arthritis rats of group M10 and group M20 after administering morphine for 7 days.The expression of EAAC1 in DRG was downregulated.Conclusion DRG EAAC1 may be involved in the mechanism of developing morphine tolerance in rats with inflammatory pain.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2010年第1期54-56,共3页
Journal of Clinical Anesthesiology
基金
天津市自然科学基金资助项目(06YFJMJC08600)
天津市卫生局科技基金资助项目(06KZ51)
关键词
慢性吗啡耐受
佐剂性关节炎
兴奋性氨基酸载体1
Chronic morphine tolerance
Adjuvant-induced arthritis
Excitatory amino acid carrier 1
