摘要
目的比较不同方法提取苦荞种子总黄酮的效果。方法分别用乙醇回流提取法、微波辅助提取法、超声波提取法、碱水浸提法及热水浸提法提取苦荞种子总黄酮,用高效液相色谱法测定总黄酮含量,计算提取产率。结果乙醇回流提取法提取总黄酮产率最高,微波和超声波提取法次之,碱水和热水浸提法最低。结论微波提取法具有明显的高效性,适于大量提取苦荞种子总黄酮。
Objective To compare the effects of different extraction methods for total flavonoids from tartary buckwheat seeds.Methods The total flavonoids were extracted from tartary buckwheat seeds by Soxhlet extraction method,microwave extraction method,ultrasonic wave method,alkali water method and hot water method,respectively.The content of total flavonoids was determined by HPLC and the extraction yield was calculated.Results The extraction yield of total flavonoids was highest by Soxhlet extraction method,second by microwave extraction method and ultrasonic wave method,lowest by alkali water method and hot water method.Conclusion The microwave extraction method is rapid and efficient,which is the appropriate method for the extraction of total flavonoids from tartary buckwheat seeds.
出处
《食品与药品》
CAS
2010年第3期93-95,共3页
Food and Drug
基金
国家自然科学基金项目(No.30671084)
