摘要
目的:探讨M蛋白致敏树突状细胞(DC)诱导激活的自体T细胞对骨髓瘤细胞特异性免疫杀伤效应。方法:取多发性骨髓瘤(MM)患者外周血单核细胞经GM-CSFI、L-4和TNF-α体外联合诱导生成DC,用DEAE-纤维素提取法自MM患者血清中分离、纯化M蛋白。以M蛋白冲击致敏DC(同时设未致敏的DC为对照),流式细胞术分析DC免疫表型,并与自体T淋巴细胞进行混合淋巴细胞反应5。1Cr释放法测定T细胞对不同靶细胞(患者骨髓瘤细胞、U266细胞)的杀伤活性。结果:经M蛋白致敏的DC激发自体T细胞增殖的能力明显强于未致敏的DC(P<0.05);同时负载M蛋白DC激活的T细胞杀伤活性也明显强于对照组(P<0.01),而两组T细胞对U266细胞均无明显杀伤活性。结论:M蛋白致敏DC能有效诱导自体T细胞对骨髓瘤细胞的特异性杀伤作用。
Objective： To investigate the anti-myeloma cells specific immune activity induced by myeloma protein-pusled dendritic cells（DCs）. Methods： DCs were obtained from peripheral blood（PB） of multiple myeloma（MM） patients after co-culture with GM-CSF,IL-4 and TNF-α. The phenotype of DCs was examined by flow cytometry. Host lymphocytes were stimulated with DCs pulsed with myeloma protein acquired from serum of MM patients on DEAE-celluose; lymphocytes treated with unpulsed-DC served as control. [^3 H]-thymidine incorporation and 4 h ^51 Cr-release assay were used to detect the proliferation and cytotoxicity against different targets（auto-myeloma cells,U266 cells） by T lymphocytes. Results： In vitro proliferation and cytotoxicity of T lymphocytes activated by myeloma protein pulsed-DCs were greater than those of T lymphocytes in the control group（P〈0.01）. T lymphocytes in neither of the 2 groups had notable cytotoxicity against U266 cells. Conclusion： DCs pulsed by myeloma protein can effectively induce specific cytotoxicity of T lymphocytes against myeloma cells.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2006年第6期624-627,共4页
Academic Journal of Second Military Medical University
基金
江苏省淮安市医学科研计划项目(WX200505)~~
