摘要
目的用HPLC法测定众生丸中三种苷类成份的含量。方法采用高效液相色谱法对众生丸中三种苷类进行含量测定,检测波长为230nm,色谱柱为Kromasil C18(4.6mm×250mm,5μm),甲醇-0.4%磷酸为流动相梯度洗脱,流速为1.0min/mL,柱温为35℃。结果芍药苷进样量在0.1542μg～2.8384μg范围内呈良好的线性关系,平均回收率为97.82%,RSD为0.99%(n=6);虎杖苷进样量在0.0976μg～1.5920μg范围内呈良好的线性关系,平均回收率为97.44%,RSD为1.31%(n=6);黄芩苷进样量在0.7692μg～11.5380μg范围内呈良好的线性关系,平均回收率为99.79%,RSD为1.75%(n=6)。结论该法简便准确,重现性好,专属性强,可作为该制剂的质量控制。
Objective To determine the three Glycosides in traditional Chinese medicine Zhongsheng Wan by HPLC. Methods The chromatographic conditions included Kromasil C18 column （250 mm×4.6 mm, 5 μm） and the mobile phase consisting of a mixture of methanol- 0.4 %phosphoric acid at a flow rate of 1.0 mL/min at 35 ℃. The detection wavelength was 230 nm. Results The linear range of paeoniflorin was from 0.154 2 μg to 2.838 4 μg, and the aver- age recovery was 97.82 %（RSD=0.99 %, n=6） . Piceid showed good linearity in the range of 0.097 6-1.592 0 μg, and the average recovery was 97.44 %（RSD=1.31%, n=6）. Baicalin showed good linearity in the range of 0.769 2- 11.538 0lμg, and the average recovery was 99.79 %（RSD=1.75 %, n=6）. Conclusion This method is simple and re- producible, and can serve as the standard for controlling the quality of Zhongsheng Wan.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2010年第2期194-197,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
