摘要
目的:研究噎膈病辨证施方在不同浓度下对食管癌细胞生长的影响;从生长信号转导角度探讨启膈散等对人表皮生长因子(hEGF)刺激的食管癌EC9706细胞生长抑制机理。方法:体外培养人表皮生长因子(hEGF)刺激的EC9706食管鳞癌细胞株,分别加入启膈散等4方提取液,通过MTT染色、流式细胞技术等观察细胞增殖变化;以western blot法检测PLC-γ1介导的生长信号通路蛋白表达。结果:启膈散(Q)、沙参麦冬汤(S)、通幽汤(T)IC50值分别为849,1004,1 615μg.mL-1;可抑制PLC-γ1信号通路相关蛋白表达,抑制作用强度:T>Q>S;结论:除补气运脾汤外,噎膈证方可抑制hEGF刺激的EC9706细胞的生长;启膈散等3方可通过抑制PLC-γ1介导的生长信号转导而抑制EC9706细胞生长。
Objective：To discuss effects of prescriptions for esophageal carcinoma in different concentrations on the proliferation of esophageal carcinoma cell line EC9706 stimulated by hEGF;to discuss inhibition mechanism of the prescriptions on esophageal carcinoma EC9706 cells stimulated by hEGF from the view of growth signal transduction.Methods： Human esophageal carcinoma cell line EC9706 cultured in vitro treated with hEGF and Qigesan（Q）,Shashenmaidongtang（S）,Tongyoutang（T） and Buqiyunpitang（B）,cells proliferation is assayed by MTT,cells proliferation and cells cycle are measured by FCM.Associated proteins within growth signal pathway mediated by PLC-γ1 were determined by western blot.Results： The half maximum inhibition rates（IC50） of Q,S and T are 849,（1 004）,（1 615） μg·mL.These 3 prescrip tions could inhibit associated proteins expression within signal pathway mediated by PLC-γ1,the effects of T and Q were better than S.Conclusion： Q S and T could inhibit cells proliferation stimulated by hEGF.The inhibition of B is very weak.The prescriptions influence EC9706 cells proliferation by inhibiting proteins expression associated with growth signal pathway mediated by PLCγ1.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第3期100-103,108,共5页
Chinese Journal of Experimental Traditional Medical Formulae
