摘要
建立了一种简便、灵敏、可重复的高效液相色谱法检测水牛血浆中吡喹酮（Praziquantel，PZQ）的含量。吡喹酮在C18（250mm×4．0mm，5μm）柱中可以很好地与杂质峰分离开。其保留时间、紫外检测波长、流动相、流速、色谱柱温度分别为10．6min、220nm、甲醇：水（70：30，V/V）、0．8mL／min和40℃。水牛血浆样品（0．5mL）经乙酸乙酯（6mL）两步提取后，采用旋转蒸发仪蒸干，1mL流动相溶解定容，取上清滤液20汕L进样做HPLC分析。吡喹酮的血浆药物浓度在0．0625～8mg／L范围内，呈现良好的线性关系，相关系数r〉0．999。在0．0625、1、16mg／L的3个药物浓度水平下，吡喹酮的平均回收率均在90％以上。日内和日间变异系数小于5％。PZQ的LOD可达0．01mg／L，LOQ为0．03mg／L。该方法的专属性强、灵敏度高，适用于水牛血浆中PZQ的药动学检测。
A simple, sensitive and reproducible method based on a reversed -phase chromatography was developed for the determination of praziquantel in water buffalo plasma. Praziquantel was separated from the impurities on a C18 column （250 mm × 4.0 mm, 5 μm） ,with retention times of 10.6 rain. Ultraviolet detection was set at 220 nm. The mobile phase consisted of methanol and distilled water （70 ： 30, V/V） , running through the column at a flow rate of 0.8 mL/min. The chromatographic analysis was operated at 40℃. Sample preparation （0.5 mL plasma） was done by a single step liquid- liquid extraction with ethyl acetate. Calibration curves in plasma at the concentrations （ 0. 062 5 - 8 mg/L） were all linear with correlation coefficients higher than 0. 999. At the fortified levels of 0. 062 5,1,16 mg/L, the mean recoveries of PZQ ranged from 90% to 120%. The precision of the method based on within - day repeatability and reproducibility （ day - to - day variation ） was below 5%. Limit of detection （LOD） was 0.01 mg/L. LOQ was 0. 03 mg/L. The method was applicable to pharmaeokinetics detection of PZQ in water buffalo plasma.
出处
《中国兽药杂志》
2010年第3期9-12,共4页
Chinese Journal of Veterinary Drug
基金
中国农业科学院上海兽医研究所资助项目
