摘要
目的利用复乳-溶剂挥发法制备脂质体,并考察其对水溶性药物氧化苦参碱的包封率。方法按文献利用高速剪切机制备水相溶有氧化苦参碱的W/O乳剂,再将此乳剂滴入水相中二次乳化并继续快速搅拌、挥干溶剂即得。并且采用超滤-HPLC法测定了脂质体的包封率。结果复乳法制备的脂质体包封率较低,绝大多数情况小于10%,当水化液的离子强度较高时包封率在15%左右,样品低温储存2周后有药物晶体析出。结论采用复乳法制得氧化苦参碱脂质体包封率较低,与文献报道的情形相符。
Objective To prepare liposomal oxymatrine by multiple emulsificatingsolvent evaporating process. Methods W/O emulsion that contained oxymatrine was prepared by employing high-speed homogenizer. Liposomal oxymatrine was obtained by adding the W/O emulsion into aqueous media dropwise and stirring the mixture vigorously until the organic solvent was evaporated thoroughly. 10 times higher of phospholipid concentration was used compared to that reported in literature. Ultrafiltratio-HPLC method is adopted to determine the encapsulation efficiency of oxymatrine liposome. Results The encapsulation efficiency was rather low by employing multiple emulsificating and solvent evaporating method that it was below 10% in most cases but some cases around 15%. Drug crystal could be found after the samples were stored 4℃ for 2 weeks. Conclusion The result is accordant with the reports in references that wate-soluble small molecular compound could be difficultly loaded in liposome with high encapsulation efficiency by the method adapted.
出处
《成都医学院学报》
CAS
2010年第1期21-24,共4页
Journal of Chengdu Medical College
基金
国家自然科学基金资助项目(30472100)
关键词
脂质体
复乳-溶剂挥发法
氧化苦参碱
大豆磷脂
包封率
liposome
multiple emulsification-solvent evaporation
oxymatrine
soybean phospholipids
encapsulation efficiency
