摘要
目的建立自制板蓝根免煎颗粒中靛玉红含量测定方法。方法用高效液相色谱法,流动相:甲醇∶氯仿(85∶15)(加0.1%三乙氨,醋酸调至中性),流速:1ml/min,检测波长:290nm,柱温:25℃。结果方法学考察合格,准确测定出自制板蓝根免煎颗粒中靛玉红含量。结论本法快速准确,可行性高。
Objective To establish a determination of indirubin content in self-made free-flying banlangen granules. Methods By HPLC, mobile phase: methanol: chloroform (85 : 15) (plus 0.1% triammonia, acetic acid to neutral), flow rate: 1 ml / rain, detection wave- length: 290 nm, column temperature: 25℃. Results Qualified methodology accurately determined indirubin content in self-made free-frying banlangen granules. Conclusion The method is rapid, accurate and highly feasible.
出处
《临床医学工程》
2010年第3期4-5,共2页
Clinical Medicine & Engineering
关键词
高效液相色谱法
板蓝根
靛玉红
High performance liquid chromatography (HPLC)
Banlangen
Indirubin