摘要
利用选择性培养基从ClO4-污染的底泥样品中分离到一株具有ClO4-还原能力的细菌JD15.对其细胞形态、结构、生理特征、16S rDNA和ClO4-还原酶α亚基基因片段pcrA的编码序列分析表明,该菌株为革兰氏阴性的短杆菌,微好氧;16S rDNA序列Dechloromonas sp.SIUL(AF170356.1)的相似性为100%,将其命名为Dechloromonas sp.JD15.该菌株的最适条件为t=25℃,pH=7;能够在ρ(ClO4-)=5×10-3～5×103mg.L-1的培养基上生长;在含0.5～10 mg.L-1的ClO4-培养基中分别培养4 d和30 d后,ClO4-的降解率分别大于50%和80%,表明该菌株可去除环境中的ClO-,具有潜在的应用价值.
By using selective culture medium,a bacterial strain JD15 which could deoxidize perchlorate was isolated from the perchlorate sewage.Based on the analysis of strain JD15 on cell shape,structure,physiochemical characteristics and sequence similarity,the strain was defined as G-,Brevibacterium with high sequence similarity of 16S rDNA and perchlorate reductase alpha subunit precursor（pcrA） gene to Dechloromonas sp.agitata（AY180108.1）.JD15 grew well under the conditions of t=25 ℃,pH=7 and ρ（ClO-4）=5×10-3～5×103 mg·L-1.When JD15 grew in medium containing 0.5~10 mg·L-1 perchlorate for 4 or 30 days,perchlorate decreased by more than 50% and 80%,respectively.The results suggest that JD15 has a potential for perchlorate removal in environment.
出处
《江苏大学学报（自然科学版）》
EI
CAS
北大核心
2010年第2期225-229,共5页
Journal of Jiangsu University：Natural Science Edition
基金
国家自然科学基金资助项目(50778083/E080402
20704020/B040305)
江苏省高等学校基金资助项目(2008CL059J)
