摘要
为了优化浸出工艺,研究浸矿体系中主要微生物种群的结构和动态变化,在槽式搅拌反应器中于45℃用中度嗜热混合菌(Acidithiobacillus caldus,Leptospirillum ferriphilum,Sulfobacillus acidophilus,Sulfobacillus thermosulfidooxidans等)浸出黄铜矿精矿,考察浸出液的pH,电极电位Eh及金属离子浓度并应用PCR-RFLP(限制性酶切片段长度多态性)方法研究细菌群落变化,浸出时间为30d。研究结果表明:Cu的浸出率为26.2%,反应器内菌种多样性不丰富,只有At.caldus和L.ferriphilum2种;群落动态变化明显,在浸出开始阶段At.caldus是优势种群,其丰度为96%,随着浸出的进行,L.ferriphilum逐渐增多,在浸出后期代替At.caldus成为优势种群,其丰度为69%。
Community succession in bioleaching process was investigated for optimization. Chalcopyrite concentrate was leached by moderately thermophilic mixed microorganisms, including Acidithiobacillus caldus, Leptospirillum ferriphilum, Sulfobacillus acidophilus, Sulfobacillus thermosulfidooxidans et al. in a stirred tank bioreactor operated at 45 ℃ for 30 d. The parameters such as pH, Eh and concentrations of metal ions were determined and synchronously the method of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism （PCR-RFLP） was used to analyze the succession of bacterial community, and their possible influences on succession of community were discussed. The results show that copper recovery is 26.2%. Only two species, i.e., At. caldus and L. ferriphilum are detected. At the beginning of bioleaching process, At. caldus is the predominant species, accounting for 96% of the whole community. During the process of leaching, L. ferriphilum gradually increases and replaces At. caldus, and accounts for 69% as the majority of community in the later stage of bioleaching process.
出处
《中南大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2010年第1期15-20,共6页
Journal of Central South University:Science and Technology
基金
国际海底区域研究开发“十一五”项目(DYXM-115-02-2-07)
海洋公益性行业科研专项经费资助项目(200805032)
教育部新世纪优秀人才支持计划项目(NECT-06-0691)
关键词
黄铜矿精矿
槽式搅拌反应器
中度嗜热菌
PCR-RFLP
群落变化
chalcopyrite concentrate
stirred tank bioreactor
moderately thermophilic microorganism
PCR-RFLP
community succession
