摘要
通过生态群落16S rRNA基因库的重建并采用限制性酶切长度多态性(RFLP)分析的方法,对铜绿山铜矿酸性矿水中微生物群落的多样性进行研究。通过RFLP对扩增出的327个16S rRNA PCR产物进行分析,对44个16S rRNA的克隆子进行测序。每个样点各有2～6个主要操作分类单元,这些操作分类单元所对应的克隆子数分别占各样点克隆子总数的56.3%～69.6%。93.8%的克隆子序列与现在的数据库中序列相似性大于90%。样点酸性矿坑水主成分分析与系统发育分析结果显示:在低pH值、高离子浓度的样点tls1与tls2中多数克隆子属于gamma-Proteobacteria(主要为Acidithiobacillus ferrooxidans)和Nitrospira科(主要为Leptospirillum);而在较高pH值、低离子浓度的样点tls3的克隆子多属于gamma-Proteobacteria(没有A.ferrooxidans)和alpha-Proteobacteria。在古矿井区域酸性矿坑水中微生物群落的多样性很低,生物地球化学特性相近的酸性矿坑水由具有相近的微生物群落组成。
The diversities of microbial community in acid mine drainage of Tonglushan copper mine were analyzed by recombining the community 16S rRNA genes library and restriction fragment length polymorphisms analyses approach. 327 amplified community 16S rRNA PCR products were analyzed by RFLP, and 44 unique 16S rRNA clones were identified. About 56.3%-69.6% of the total number of 16S rRNA clones in the clone library of each was affiliated with 2-6 dominant operational taxonomic units. The similarity for 93.8% of the sequences of the unique 16S rDNA clones was greater than 90% with those in current database. The principal-components analysis of the biogeochemical properties and phylogenetic analysis show that most of the bacterial clones are affiliated with members of the gamma-Proteobacteria family （mainly Acidithiobacillus ferrooxidans） and the Nitrospira family （mainly Leptospirillum） in tlsl and tls2 where the concentrations of ions are high, while those are affiliated with members of the gamma-Proteobacteria family （no Acidithiobacillus ferrooxidans） and the alpha-Proteobacteria family in t1s3 where concentrations of ions are very low. The diversity of microbial community in acid mine drainage is at low level and samples with similar biogeochemical properties have similar microbial communities.
出处
《中南大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2007年第3期414-420,共7页
Journal of Central South University:Science and Technology
基金
国家自然科学基金资助项目(50374076)
国家自然科学基金创新群体基金资助项目(50321402)
国家重点基础研究发展规划项目(2004CB619201)
关键词
酸性矿坑水
古矿井
微生物群落
多样性
限制性酶切片断多样性
生物地球化学
acid mine drainage
ancient mine
microbial community
diversity
restriction fragment length polymorphisms
biogeochemistry
