摘要
质谱技术已经成为目前蛋白质鉴定的重要工具。定量分析细胞内蛋白质组的动态变化,是当前研究蛋白质功能、揭示细胞生物机理、寻找疾病蛋白标记物和药物靶标的迫切需要。本文综述了基于质谱技术蛋白质定量的策略、方法和应用等方面近年来的进展,评述了几种蛋白质质谱定量方法的特点和应用潜力。
Mass spectrometry has become a powerful technique for proteomics analysis. It would be highly desirable to obtain exact quantitative values of each protein in a system,and these dynamic protein data obtained from cell can provide important information to study protein function,reveal the biological process and discover protein biomarker and drug target. Here,we report the development of quantitative mass spectrometry-based proteomics on strategy,method and application in recent years and further it is addressed for their characteristics and potentialities in protein analysis.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第3期434-441,共8页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(Nos.90919008
20975053
20845004)
国家973计划(No.2007CB914100)
NN-CAS-2008-8项目资助
关键词
质谱
蛋白质组学
定量
同位素标记
评述
Mass spectrometry
Proteomics
Quantitative
Isotope labeling
Review
