摘要
通过实验比较了毛细管区带电泳(CZE),毛细管凝胶电泳(CGE)及聚丙烯酰胺平板凝胶电泳(SDS-PAGE)在分离、定量牛奶蛋白上的效果比较。结果表明,在分离的效果及重现性上,CZE,尤其是用带有聚乙烯醇(PVA)涂层的毛细管,酸性的电泳缓冲液和低离子强度的样品缓冲液要优于其他方法,可用于快速分离、定量牛奶蛋白。
A method for the identification and quantification of major bovine milk proteins was developed using capillary zone electrophoresis （CZE）. This method included the use of a polyvinyl alcohol （PVA） coated capillary column, a running buffer containing 6 M urea at pH 3, and a sample buffer containing 7 M urea and pH 7.5 adjusted with bis-tris-propane, Compared with slab polyacrylamide gel electrophoresis （PAGE） and capillary gel electrophoresis （CGE）, this method was found to be more rapid, reproducible and sensitive in quantification and identification of milk protein.
出处
《中国乳品工业》
CAS
北大核心
2005年第8期51-54,57,共5页
China Dairy Industry
