摘要
目的探讨选择性环氧化酶-2抑制剂塞来昔布对膀胱肿瘤的抑制作用及其机制。方法建立小鼠膀胱肿瘤模型并随机分为对照组、实验1组和实验2组，分别给予生理盐水和不同剂量的塞来昔布灌胃，观察肿瘤的生长情况，测量肿瘤体积、质量并计算抑瘤率；免疫组织化学方法检测肿瘤原癌基因bcl-2和半胱氨酸天门冬氨酸蛋白酶-3（caspase-3）的表达情况，并计算其免疫组化评分（HIS）。结果实验1、2组肿瘤的平均质量明显低于对照组，差异有显著性（F=64．99，q=4．52、15．66，P〈0．05）。实验1、2组的抑瘤率分别为43％和58％。3组间bcl-2的HIS比较差异有显著性（F=25．08，q=3．75～9．91，P〈0．05）；3组间caspase-3的HIS比较差异有显著性（F=16．82，q=3．26～8．15，P〈0．05）。结论塞来昔布能抑制小鼠膀胱肿瘤的生长，其机制可能与抑制bcl-2、增强caspase-3的表达有关。
Objective To investigate the depressant effect of celecoxib, a cyclooxygenase-2 inhibitor, on the growth of urinary bladder cancer in mice. Methods A model of bladder tumor in mice was created and randomized to control group, experiment groups I and II. An intragastric administration of normal saline and different doses of eelecoxib was given to different groups. The growth of tumor was observed, the gross tumor volume and weight measured, and tumor-inhiblting rate calculated. The expressions of bcl-2 and caspase-3 proteins were examined immunohistochemically and immunohistochemical scores calculated. Results The average volume and weight of tumors in experiment groups I and II were significantly lower than those of the control group （F=64.99;q=4.52,15.66;P〈0.05）, the inhibition rate of the two groups was 43% and 58%, respectively. The differences in the scores of bcl-2 expressions among the experiment groups I , Ⅱ and the control group were significant （F= 25.08;q=3.75-9. 91 ;P〈0.05）, and that of caspase-3 among the three groups were also significant （F=16.82;q=3.26-8.15; P〈0.05）. Conclusion Celecoxih can suppress the growth of bladder tumor in rats, its mechanism might be associated with decreasing the expression of bcl-2, and increasing that of caspase-3.
出处
《齐鲁医学杂志》
2010年第2期104-106,共3页
Medical Journal of Qilu
