摘要
为研究硝酸铈对盾叶薯蓣快速繁殖的影响，采用以盾叶薯蓣茎段为外植体，在其愈伤诱导培养基、丛生芽培养基及生根培养基中添加不同浓度的Ce3＋结果表明，含Ce3＋为5mg／L的培养基可显著提高愈伤组织诱导率，含Ce3＋1mg／L的培养基丛生芽诱导率最高；1—15mg／L的Ce3＋对盾叶薯蓣组培苗生根有明显的促进作用，5mg／L的Ce3＋显示出最强的促进效应．可以认为，一定浓度Ce3＋对诱导愈伤组织生长、丛生芽萌发及不定根生长有促进作用，但高浓度的Ce3＋均对其呈现抑制效应．
To study the effect of rare earth Ce（ NO3 ）3 on rapid clonal propagation of Dioscorea zingiberensis. Using stems of Dioscorea zingiberensis as explants, different concentrations of Ce （NO3 ）3 were supplemented to the medium （1/2 MS ＋ 0. 5 μmol/L IBA） to study the effect of Ce3 ＋ on the callus induction medium, adventitious bud initial medium and adventitious root medium. The callus induction was stimulated by 5.0 mg/L Ce3 ＋ ; 1.0 m g/L Ce3 ~ stimulated the bud initial induction; while 1 -15 mg/L stimulated adventitious root. The callus induction adventitious bud initial and roo- ting was stimulated by the low concentrations of rare earth elements, but higher concentration inhibited them.
出处
《南京师大学报（自然科学版）》
CAS
CSCD
北大核心
2010年第1期94-97,共4页
Journal of Nanjing Normal University(Natural Science Edition)
基金
科技部项目(2003DEB6J074)
江苏省科技厅项目(BG2001045)
关键词
铈
盾叶薯蓣
愈伤组织
不定根
cerium, Dioscorea zingiberensis, callus, adventitious root
