摘要
目的明确免疫大鼠血清中抗β1、β3肾上腺素受体及M2乙酰胆碱受体抗体的IgG亚类，为进一步研究此类抗体的作用机制提供实验依据。方法以合成的大鼠β1、β3肾上腺素受体和M2乙酰胆碱受体细胞外第二环肽段为抗原分别主动免疫Wistar大鼠，应用免疫球蛋白纯化技术特异性提纯其血清中IgG类抗体，应用BCA进行蛋白定量，调整血清中蛋白量一致，用SA—ELISA法检测抗β1、β3肾上腺素受体和M2乙酰胆碱受体抗体的IgG亚类。结果免疫组中属于IgG2a亚类的抗β1肾上腺素受体抗体的A值为（0．45±0．01），明显高于对照组（0．08±0．003，P〈0．05）；免疫组中属于IgG2b亚类的抗艮肾上腺素受体抗体的A值为（0．59±0．02），显著高于对照组（0．2±0．02，P〈0．05）；免疫组中属于IgG2a亚类的抗M2乙酰胆碱受体抗体的A值为（0．56±0．04），显著高于对照组（0．12±0．01，P〈0．05）。结论免疫大鼠血清中抗β1肾上腺素受体抗体和抗M2乙酰胆碱受体抗体主要属于IgG2a亚类，抗β3肾上腺素受体抗体主要属于IgG2b亚类。
Objective To detect the IgG subclass of autoantibodies against β1/β3 adrenoceptor or M2- muscarinic receptor in the sera of rats. Methods Adult Wistar rats were actively immunized with the synthetic peptide corresponding to the sequences of the second extracellular loop of β1/β3 adrenoceptor or M2-muscarinic receptor respectively. The autoantibedies that belong to IgG immunoglobulin were purified, and then the IgG subclass of autoantibodies against β1/β3 adrenoceptor or M2-muscaxinic receptor were detected by SA-ELISA. Results The A value of IgG2a subclass of autoantibedy against β1 adrenoceptor in the immunized rats was 0.45 ±0.01, which was significantly higher than that in the control group of the same period （A value 0.08±0.003, P〈0.05）. The A value of IgG2b subclass of autoantibedy against β3 adrenoceptor in the immunized rats was 0.59±0.02, which was significantly higher than that in the control group of the same period （A value 0.2±0.02, P〈0.05）. The A value of / IgG2a subclass of autoantibody against M2-muscarinic receptor in the immunized rats was 0.56±0.04, which was significantly higher than that in the control group of the same period （A value 0.12±0.01, P〈0.05）. Conclusion The main IgG subclass of autoantibodies against β1 adrenoceptor or M2-muscarinic receptor in the immunized rats is IgG2a subclass; the main IgG subclass of autoantibodies against β3 adrenoceptor in the immunized rats is IgG2b subclass.
出处
《中国心血管病研究》
CAS
2010年第3期218-221,共4页
Chinese Journal of Cardiovascular Research
基金
国家自然科学基金资助项目（30670835）
