摘要
在营养液中加入不同浓度硅且接种黄瓜霜霉病菌后,通过调查其病情指数和检测黄瓜叶片内硅元素含量及过氧化物酶(guaiacol-peroxidase,POD)、多酚氧化酶(polyphenol oxidase,PPO)、苯丙氨酸解氨酶(phenylalanine ammonia-lysae,PAL)、β-1,3葡聚糖酶(β-1,3-glucanase)、超氧化物歧化酶(superoxidedismutase,SOD)5种抗霜霉病相关酶活性,探讨硅抑制黄瓜霜霉病的生理生化机制。结果表明,营养液中硅浓度为100mg/L的处理,黄瓜霜霉病病情指数为21.3,防治效果达到62.8%;营养液中硅浓度与黄瓜叶片内硅元素含量呈正相关,200mg/L处理叶片内硅元素含量最高,且7天后达到2.98mg/g;加硅处理接种黄瓜霜霉病菌后,黄瓜叶片抗病相关酶活性变化明显且差异达显著水平,其中硅浓度为100～200mg/L时上述5种酶活性最高。
Cucumber seedlings were treated with different concentrations of sodium metasilicate （Si） solutions by supplying Si into nutrient solution, and inoculated with Pseudoperonospora cubensis or not. The effects of Si on disease index and major defense-related enzymes activities （ guaiacol-peroxidase, polyphenol oxidase, phenylalanine ammonia-lysae, β-1, 3-glucanase and superoxide dismutase） were investigated. The results showed that the treatment with the lowest disease index （21.3） and the highest efficacy （62.8%） of downy mildew control was at 100 mg/L concentration. The supply of Si significantly increased the Si contents in cucumber leaves, and Si showed the largest amount of 2.98 mg/g when applied at 200 mg/L concentration 7 days later. The supply of Si resulted in significant changes of defense-related enzymes activities in cucumber after P. cubensis infection, and the treatments with the highest enzymes activities at 100 -200 mg/L concentration. The above results suggested that the concentration of Si played an important role in inhibiting P. cubensis and the changes of major defense-related enzymes activities.
出处
《植物保护学报》
CAS
CSCD
北大核心
2010年第1期37-41,共5页
Journal of Plant Protection
关键词
硅
黄瓜霜霉病
黄瓜霜霉病菌
抗病相关酶
植物抗性
silicon
cucumber downy mildew
Pseudoperonospora cubensis
defense-related enzyme
plant-defense
