摘要
以滤纸作为唯一碳源,成功从人体肠道微生物中筛选分离出1株产纤维素酶的菌株H1。分离富集后的菌株用纤维素刚果红培养基进行筛选鉴定、革兰色染色、对菌株进行分类并对该菌种进行CMC酶活测定与分析、分子鉴定。H1在纤维素刚果红平板上能呈现阳性反应;镜检发现,H1菌株为短杆状,革兰色阴性菌;该菌株在13h酶活最大,为0.8U左右;分子鉴定结果为假单孢菌属。
Cellulase-secreting microorganisms include fungi and a few cellulose-decomposing bacteria. In this study, human rectal microbiota were screened by using filter paper as the only carbon source, and one cellulase-secrering bacteria, H1, was successfully separated. Its cellulase-secreting activity was identified using cellulose congo-red medium, morphology identification undertaken by gram staining, and classification accomplished via molecular identification. The cellulase activity of H1 was also analyzed. The results show it is positive in the cellulose congo-red medium, short and rod-shaped and gram-negative. Cellulase activity analysis indicated that its highest cellulase activity is about 0.8 U occurred at 13 hours. Molecular identification characterized the strain as Pseudomonas specie.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2010年第1期49-51,共3页
Food and Fermentation Industries
基金
暨南大学青年基金(51208029)资助项目
