摘要
目的建立HPLC法同时测定大黄牡丹汤中大黄酸、丹皮酚和芍药苷的含量。方法采用KromasilC18色谱柱(250mm×4.6mm,5m),流动相:乙腈-体积分数为0.1%的磷酸水溶液梯度洗脱,流速:1.0mL·min-1,检测波长:230nm,进样量:10L。结果大黄酸的线性为1.61～16.1mg·L-(1r=0.9997),平均回收率为98.0%,RSD为0.7%;丹皮酚的线性为1.64～16.4mg·L-(1r=0.9997),平均回收率为99.8%,RSD为1.3%;芍药苷的线性为1.54～15.4mg·L-1(r=0.9996),平均回收率为100.3%,RSD为1.9%。结论该方法能更加全面地控制该方的质量,优化后的提取工艺可为今后剂型的改进奠定基础。
Objective To determine 3 components in decoction of Rheum and Moutan by HPLC simultaneously. Methods The seperation of 3 ingerdients was achieved on a Kromasil C18 column（250 min×4.6 mm,5μm） with gradient elution using solvents of acetonitrile 0.1% phosphoric acid solution. The speed of flowing phase was 1.0 mL·min^-1. The detection wavelength was at 230 nm. Results The linear range was 1.61-16.1 mg·L^-1 for rhein, 1.64-16.4 mg·L^-1 for paeonol and 1.54-15.4 mg·L^-1 for paeoniflorin. Average recoveries of samples were all over 98.0%, RSD were all below 2.0%. Conclusion The method was simple, accurate and can be used as assessment about quality of Decoction of Rheum and Moutan.
出处
《中国药物警戒》
2010年第3期139-141,共3页
Chinese Journal of Pharmacovigilance
关键词
高效液相色谱法
大黄牡丹汤
有效成分
High Performance Liquid Chromatography-Fluorescence（HPLC）
decoction of Ikheum and Moutan
main gredients
