摘要
目的:探讨大黄牡丹汤对TNBS诱导的小鼠实验性结肠炎的治疗作用及作用机理。方法:采用三硝基苯磺酸(TNBS)法制作实验性结肠炎小鼠模型,给予大黄牡丹汤治疗,观察小鼠的一般状态和DAI评分、结肠组织学变化。采用Luminex液相芯片系统检测血清中白介素1β、白介素4和肿瘤坏死因子α的含量。结果:大黄牡丹汤对TNBS结肠炎小鼠的一般状况及DAI评分有改善作用、并能缓解结肠局部的炎症,可降低血清中白介素1β和肿瘤坏死因子α的含量的水平。结论:大黄牡丹汤具有一定地防治TNBS所诱导的小鼠结肠炎的作用,其机制可能与抑制白介素1β和肿瘤坏死因子α的分泌有关。
Objective： To study the effect of Dahuang Mudan Decoction on experimental colitis induced by trinitrobenzene sulfonic acid in mice. Methods： Mice colitis model was established by rectal administration of trinitrobenzene sulfonic acid （TNBS）. The changes in disease activity index （DAI） and histologic scores were assessed. The concentrations of TNF-α, IL-1β and RM in the blood serum were detected by multiplexed microsphere based flow assay. Results： Dahuang Mudan Decoction therapy significantly reduced DAI and histological scores in mice, and alleviate inflammation in colonic tissue. TNF-α and IL-1β levels significantly decreased in mice serum after being treated with Dahuang Mudan Decoction. Conclusions： Dahuang Mudan Decoction showed positive therapeutic efficacy on mice colitis, and its mechanisms maybe involve inhibiting the production of IL-1β and TNF-α.
出处
《现代生物医学进展》
CAS
2007年第12期1791-1793,共3页
Progress in Modern Biomedicine
基金
国家中医药管理局课题(No 02-03ZL46)
广州中医药大学创新基金课题(No K0050040)
关键词
大黄牡丹汤
结肠炎
液相芯片
细胞因子
Dahuang Mudan Decoction
Colitis
Multiplexed microsphere based flow assay
Cytokine
