摘要
本文用间接免疫荧光染色流式细胞计数分析技术和LDH释放法检测了11例肝癌患者肿瘤浸润性淋巴细胞（TIL）的细胞表型及杀伤活性。结果培养初期TIL细胞特征是以CD3为主，CD4／CD8为0．83，NK细胞23．3±3．6％。经重组白细胞介素2（rIL—2）激活20天后，CD3减少，CD4／CD8为1.85，NK细胞数量增至39．2±9．8％，P＜0．01。激活后的TIL对自体肝癌细胞杀伤率比培养初期高3．74倍，亦比对同时培养的BEL—7204肝癌细胞杀伤率高1．75倍。对自体和异体肝癌细胞杀伤作用均显著高于LAK细胞，且具有靶细胞特异性。结果表明肝癌TIL对肝癌细胞杀伤作用，可能与NK细胞数量增多，导致肝癌细胞凋亡有关。
Changes of phenotype and antitumour activity of tumour-infiltrating lymphocyte(TIL) fromcancer tissue of 11 patients with primary hepatocellular carcinoma were observed with indirectimmnofluofluorecent straning and flow cytometry technique. The results showed that the phenotype of TIL after induction by rll-2 were CD3 51. 9±13. 7%. CD4 33. 7±9. 2% . CDS 18. 3±8. 2% .CD20 6. 8±3. 1 % and NK cell 39. 2±9. 8%. NK cell was markedly higher than that beforeinduction. It was demonstrated that antitomour activity of TIL was at the highest level in 20 days.This acitivy of TIL to autologous cancer cells and 7204 cancer cells was significant higher than thatLAK cells. It was suggested that effects of antitomour of TIL may be related to increasing numberof NK cells,then the latter might cause hepatocellular carcinoma cells apoptosis.
出处
《临床肝胆病杂志》
CAS
北大核心
1998年第4期212-214,共3页
Journal of Clinical Hepatology
基金
吉林省科委应用研究计划资助
关键词
肝肿瘤
肝癌
肿瘤浸润
淋巴细胞
细胞表型
hepatocellular carcinoma TIL phenotype antitumour activity NK cell
