摘要
目的研究缺氧诱导因子1α(HIF-1α)的常见多态性C1772T与食管鳞癌临床病理特征的关系。方法用PCR-RFLP法研究95例食管鳞癌、104例健康对照的HIF-1α的常见多态性C1772T的基因型(突变者经直接测序确认);免疫组化法测定不同基因型肿瘤组织中HIF-1α的表达;比较不同基因型肿瘤的HIF-1α的表达水平、肿瘤大小、淋巴结转移、TNM分期的差异。结果在食管鳞癌组和健康对照组中,只发现C/C和C/T两种基因型,食管鳞癌组C1772T的突变率为11.58%(11/95),健康对照组为10.58%(11/104),两组差异无统计学意义;食管鳞癌组中C/T和C/C亚组比较,HIF-1α表达无差异。在肿瘤浸润深度匹配的情况下,C/T亚组肿瘤较大、淋巴结转移率更高。结论HIF-1α基因的多态性C1772T与食管鳞癌的肿瘤大小和淋巴结转移有关。
Objective This study is designed to investigate the common single nucleotide polymorphism （C1772T） of hypoxia-inducible factor gene and its impacts on clinicopathological features of esophageal squamous cell carcinoma （ESCC）. Methods Genotype of C1772T of ninety-five cases of ESCC patients and 104 healthy controls were studied by PCR-RFLP. Mutation was confirmed by direct DNA sequencing. The impacts of C1772T genotypes on expression of tumor size, invasive depth, lymph node metastasis, distant metastasis, histological grade and TNM stage of ESCC were studied. Results The genotype frequency observed in the patients and controls was 11.58% versus 10. 58% respectively （P 〉 0. 05） for genotype C/T. Genotype T/T was not found in this study. Significant difference was found in tumor size and lymph node metastasis between C/T and C/C groups. Conclusion C1772T genotype is associated with larger tumor and higher rate of lymph node metastasis.
出处
《临床肿瘤学杂志》
CAS
2010年第2期136-138,共3页
Chinese Clinical Oncology
关键词
缺氧诱导因子1Α
单核苷酸多态性
食管鳞癌
临床病理特征
Hypoxia-inducible factor 1α（ HIF--1α）
Single nucleotide polymorphism
Esophageal squamous cell carcinoma
Clinicopathological features
