摘要
目的:研究血管内皮生长因子C(VEGF-C)及其受体VEGFR-3在食管鳞癌组织和癌旁组织中的表达,并分析他们与肿瘤病理分级、淋巴结转移等临床病理特征之间的关系.方法:运用免疫组织化学法检测37例食管鳞癌组织和12例癌旁组织中VEGF-C和VEGFR-3的表达情况,并对阳性表达VEGFR-3的组织进行染色管腔计数结果:37例食管鳞癌组织和12例癌旁组织中VEGF-C的阳性表达率分别为43.24%(16/37)和7.69%(1/12),二者有显著性差异(P<0.05).在肿瘤组织中vEGF-C的表达与淋巴结转移和肿瘤浸润深度显著相关(P=0.000,P= 0.026),而与患者年龄、肿瘤大小和肿瘤分级无明显相关性;VEGF-C阳性组的VEGFR-3染色脉管计数较VEGF-C阴性组高,二者有明显相关性(5.50±1.37/HPF vs 2.81±1.12/HPF,P<0.05);淋巴结转移组VEGFR-3阳性染色脉管计数较无转移组的计数高(5.60±1.45/HPF vs 2.86±1.04/HPF,P<0.001).结论:VEGF-C在食管鳞癌组织中的表达明显高于癌旁组织,且与淋巴结转移和肿瘤浸润深度有相关性.VEGF-C和VEGFR-3可能介导食管鳞癌中脉管的生成,并参与肿瘤的淋巴结转移.
AIM： To investigate the expression of vascular endothelial growth factor-C （VEGF-C） and its receptor VEGFR-3 in esophageal squamous carcinoma and para-tumorous tissue samples and the relationship between VEGF-C and VEGFR-3.
METHODS： Expression of VEGF-C and VEGFR-3 was tested by immunohistochemistry in 37 esophageal squamous carcinoma and 12 paratumorous tissue samples. The number of stained lymphatic vessels in VEGFR-3 positive tissue samples was recorded.
RESULTS： The positive expression rate of VEGF-C was 43.24% in esophageal carcinoma tissue samples （16/37）, which was much higher than that in para-tumorous tissue samples （7.69%, P 〈 0.05） and correlated to lymph node metastasis and depth of tumor invasion （P = 0.000, P = 0.026）, but not to the patient age, tumor size or differentiation. Immunohistochemistry showed that the mean number of VEGFR-3 in stained lymphatic vessels of the VEGF-C positive group was higher than that in the VEGF-C negative group （5.50 ± 1.37/HPF vs 2.81 ± 1.12/ HPF, P 〈 0.05）. The mean number of VEGFR-3 in stained lymphatic vessels in patients with lymph node metastasis was higher than that in those without metastasis （5.60 ± 1.45/HPF vs 2.86 ± 1.04 /HPF, P 〈 0.001）.
CONCLUSION： The positive expression rate of VEGF-C is higher in carcinoma tissue samples than in para-tumorous tissue samples. Expression of VEGF-C is closely related with lymph node metastasis and depth of tumor invasion. VEGF-C and VEGFR-3 may promote the growth of canalis haemalis and metastasis of esophageal squamous carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第4期431-435,共5页
World Chinese Journal of Digestology
关键词
血管内皮生长因子C
血管内皮生长因子
受体3
食管鳞癌
免疫组织化学
Vascular endothelial growth factor-C
Vascular endothelial growth factor-receptor
Esophageal squamous carcinoma
Immunohistochemistry
