摘要
目的：研究神经再生素对体外培养神经干细胞分化的促进作用及对其生长相关蛋白（GAP43）、神经丝蛋白（NF-H）表达的影响。方法：取出生3～5d的新生SD大鼠大脑皮层进行神经干细胞的体外培养、鉴定，神经干细胞与0、1、2mg／L的神经再生素（NRF）共培养8d，相差显微镜观察分析，应用Real-timePCR对与不同浓度的NRF（0、1、2、4、8rag／L）共培养8d的神经干细胞进行GAP43、NF-H的表达量检测。结果：成功培养出具有多向分化潜能的神经干细胞；神经再生素可明显促进神经干细胞的分化，并能在一定范围内随浓度递增而有效促进GAP43、NF-H的表达，且最佳作用浓度为4mg／L。结论：神经再生素可以促进神经干细胞的生长和分化。
Objective： To determine the effects of nerve regeneration factor （NRF） on neural stem cells growth and differenti- ation in vitro and expression of growth associated protein 43 （GAP-43） and neurofilament-H （NF-H）. Methods： The neural stem cells from the cerebral cortex of new-born 3 - 5 d rats were cultured and identified. After different periods incubation with different NRF concentrations （0, 1 and 2 mg/L）, the neural stem cells were observed and photographed by phase con- trast microscopy. Then, the differentiation percentage and processes length were analyzed by using the Scion software. Real- time fluorescence quantitative RT-PCR was performed to examine the mRNA levels of GAP-43 and NF-H after 8-day incuba- tion with different concentrations of NRF （0, 1, 2, 4 and 8 mg/L）. Results： Neural stem cells were successfully obtained. NRF obviously promoted the differentiation in cultured neural stem cells. Real-time fluorescence quantitative RT-PCR showed that the mRNA level of GAP-43 and NF-H was up-regulated by NRF in a dose-dependent manner, with the best concentration of 4 mg/L. Conclusion： NRF could promote the neural stem cells growth and differentiation.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2010年第1期6-9,13,共5页
Chinese Journal of Anatomy
基金
国家973计划项目资助
关键词
神经再生素
神经干细胞
分化
nerve regeneration factor
neural stern cells
differentiation
