摘要
目的探讨八面蒙脱石对急性坏死性胰腺炎（ANP）大鼠回肠黏膜NF—KB活化的影响。方法120只雄性sD大鼠按完全随机法分为假手术组、ANP组、莫沙必利组、八面蒙脱石组和莫沙比利＋八面蒙脱石（联合）组，每组24只大鼠。采用胰胆管内逆行注射牛磺胆酸钠诱导大鼠ANP模型；假手术组开腹注射生理盐水后关腹。各治疗组于造模前30min分别给予相应药物灌胃1次。术后3、6、12h分批处死动物。取胰腺组织行病理检查；取血测定TNF-α、IL-1水平；取回肠观察超微结构的改变；免疫组化法检测回肠黏膜NF—KB的表达；Western blotting法检测回肠组织IKBα水平。结果与假手术组比较，ANP组胰腺损伤明显，血TNF—α、IL-1水平明显升高[（36．79±1．14）pg/ml对（9．00±0．01）pg／ml，（25．17±1．93）ng／ml对（3．45±0．11）ng／ml，P值均〈0．05]，回肠绒毛高度显著下降[（0．25±0．22）×10^-6m对（1．50±0．33）×10^-6m，P〈0．05]，柱状细胞指数显著降低[（2．40±1．65）×10^6个／m对（13．5±4．28）×10^6个／in，P〈0．05]，NF—kB表达增加（6．92±1．54对1．28±0．29，P〈0．05），IKBα蛋白水平下降（3．30±1．99对24．32±1．93）。莫沙必利组和八面蒙脱石组的各项指标与ANP组均无显著差异。联合组的血TNF-α、IL-1水平[（30．57±0．39）ps／ml和（13．45±1．26）ng／ml]、回肠绒毛高度和柱状细胞指数[（1．05±0．28）×10^-6m和（10．10±2．50）×10^6个／m]、NF—kB活化（4．32±1．57）和IKBα蛋白水平（15．99±1．26）均与ANP组有统计学差异（P值均〈0．05）。结论八面蒙脱石联合莫沙必利灌胃能减少ANP大鼠回肠黏膜NF—KB的大量激活，减轻胰腺和回肠黏膜的损伤程度。
Objective To investigate the role of octahedral montone on ileum NF-KB activation in rats with acute necrotizing pancreatitis （ANP）. Methods 120 SD rats were randomly divided into sham-operation group （A） , ANP group （B） , mosapride treatment group （C） , montone treatment group （D） , montone and mosapride treatment group （E）. Retrograde pancreatic duct injection of sodium taurocholate was used to establish ANP models, octahedral montone and mosapride were given by intragastric feeding 30 min before ANP model induction, group A was injected NS after opening the abdomen. Pancreatic tissue injury was evaluated; the serum level of TNF-α and IL-1 was determined ; the ultrastructural changes of the ileum was observed. The expression of NF-KB in the ileum mucosa was detemined by immunohistochemistry. The expression of IKBα protein in the ileum tissue was determined by Western blotting. Results Compared with group A, pancreatic tissue injury was more severe in group B, and serum level of TNF-α and IL-1 was much higher in group B [ （36.79 ± 1.14 ） vs （9. 00± 0. 01 ） pg/ml, （25.17 ± 1.93 ） vs （ 3.45 ± 0. 11 ） ng/ml, P 〈 0. 05 ] ; the height of the villi was significantly lower [ （ 0. 25 ± 0. 22 ） × 10^ -6 m vs （ 1.50 ± 0. 33 ） × 10 ^-6 m, P 〈 0. 05 ） ], the index of columnar cell was significantly lower [ （ 2.40 ± 1.65 ） × 10 ^-6 m vs （ 13.5 ± 4.28 ） × 10^6/m, P 〈 0 . 05 ） ] ; the expressions of NF-KB was significantly eleveted （ 6.92 ± 1.54 vs 1. 28 ± 0.29, P 〈 0.05 ）, the expressions of IKBα protein was significantly decreased （3.30 ± 1.99 vs 24.32 ± 1.93）. There was no significant difference between group D and C. The serum level of TNF-α and IL-1 [ （30.57 ± 0.39）pg/ml and （13.45 ±1.26）ng/ml ], the height of the villi and the index of columnar cell [ （ 1.05 ± 0.28 ） × 10^-6m and （ 10.10 ± 2.50） × 10^6/m and the expressions of NF-kB （4. 3240 ± 1. 5657） and IKBα （ 15. 987 ± 1. 262） was statisti
出处
《中华胰腺病杂志》
CAS
2010年第1期47-50,共4页
Chinese Journal of Pancreatology
