摘要
[目的]研究不同碳源种类对中国李"海湾红宝石"离体培养的影响。[方法]以3年生"海湾红宝石"李新稍茎段为试材,在初代培养和生根培养中添加蔗糖、葡萄糖、山梨醇、果糖4种碳源进行外植体接种,30、60d后分别统计增殖倍数、新梢率和生根率。[结果]在中国李增殖、诱导过程中,葡萄糖(30g/L)作碳源明显促进增殖和生长。生根诱导过程中,采用蔗糖(15g/L)为碳源,外植体生根率和生根倍数均最高。[结论]初代培养和生根培养中分别选用葡萄糖和蔗糖作碳源,中国李培养效果好。
[Objective]The research aimed to study the effects of different carbon sources on in vitro culture of Chinese plum（Prunus salicina）.[Method]With the stems of 3-year-old Chinese plum as tested materials,sucrose,glucose,sorbitol and fructose were added into multiplication culture and rooting culture,respectively.Four kinds of carbon sources were used to inoculate explants.And the multiplication times new shoot ratio and rooting rate were counted after 30 and 60 days respectively.[Result]30 g/L glucase as carbon source during the multiplication and induction period of Chinese plum could obviously promote the multiplication and growth.During the rooting induction period,the rooting rate and rooting times were the highest when 15 g/L sucrose was used as carbon source.[Conclusion]Glucose as carbon source during multiplication culture and sucrose as carbon source during rooting culture showed better effects in the culture in vitro of Chinese plum.
出处
《安徽农业科学》
CAS
北大核心
2010年第4期1720-1720,1776,共2页
Journal of Anhui Agricultural Sciences
基金
长江大学硕士科研启动基金项目(39210267)
关键词
李
离体培养
葡萄糖
蔗糖
Plum
Culture in vitro
Glucose
Sucrose
