摘要
目的探索转化生长因子-β1(transforming growth factors-β1,TGF-β1)和热休克蛋白47(heat shock protein,HSP47)在臀肌挛缩带中的表达情况及其对臀肌挛缩症(gluteal muscle contracture,GMC)发病的作用。方法收集臀肌挛缩症患者挛缩带和病变旁肌肉的病理标本,运用逆转录-聚合酶链式反应(RT-PCR)、免疫组化和Westernblot技术检测TGF-β1和HSP47在mRNA水平和蛋白水平的表达情况。结果TGF-β1和HSP47定位于成纤维细胞和血管内皮细胞的胞质中,并且呈强阳性表达,其在mRNA水平分别上调了8.1和3.6倍(P<0.05)。蛋白水平的表达情况与mRNA一致,分别上调11.2倍和7.6倍(P<0.05)。结论TGF-β1和HSP47的表达上调可能是引起挛缩带产生的重要机制。
Objective To explore the expression and effect of transforming growth factors-β1（TGF-β1） and heat shock protein（HSP47） on gluteal muscle contracture（GMC）.Methods We collected contraction band and adjacent muscle from GMC patients and explored the expression of TGF-β1 and HSP47 using immunohistohemistry,reverse transcription and polymerase chain reaction（RT-PCR）,and Western blot analysis.Results TGF-β1 and HSP47 were intensely expressed in fibroblast cells and vascular endothelial cells.The expression of them increased 8.1-fold and 3.6-fold at the mRNA level,respectively（P〈0.05）.The same changes were found at the protein level,which increased 11.2-fold and 7.6-fold,respectively（P〈0.05）.Conclusion Up-regulation of TGF-β1 and HSP47 may initiate fibrotic cascade in the gluteal muscles of GMC patients.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2010年第2期221-223,238,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省食品药品监督管理局臀肌挛缩症专项基金(No.Y200334002)
