摘要
目的:测定中药栀子中栀子苷的含量。方法:采用高效毛细管电泳法,电泳条件为石英毛细管柱(50μm×83cm,实际长度74cm),运行缓冲液为0.05mol.L-1Na2B4O7:0.01mol.L-1Na2HPO4=5∶1(16℃pH=9.2),分离电压为28kV,压力进样30mbar×8s,温度为25℃,检测波长为238nm。结果:栀子苷在0.5～1.75mg.mL-1范围内呈良好的线性关系(r=0.9998),平均回收率为99.64%,RSD为1.23%。结论:高效毛细管电泳法可用于测定栀子中栀子苷的含量。
Objective：To determine the content of Geniposide Baicalin in Fructus gardeniae.Methods：Apply the methods of HPCE,and the best condition was using the quartz capillary tube（50μm×83cm,practical length is74cm）,0.05mol·L-1Na_2B_4O_7：0.01mol·L-1Na_2HPO_4=5∶1（pH=9.2 at 16℃）buffer,pressure injection 30mbar×8s,at a constant voltage of 28kV and temperature at 25℃.It was detected on 238nm.Results：Geniposide Baicalin had a good linearity within 0.5～1.75mg·mL-1（r=0.9998）,and the average recovery were 99.64% with RSD 1.23%.Conclusion：This method is feasible for determination of Geniposide Baicalin in Fructus gardeniae.
出处
《辽宁中医杂志》
CAS
北大核心
2010年第2期318-319,共2页
Liaoning Journal of Traditional Chinese Medicine
基金
国家重点基础研究发展计划(973计划)资助项目(2007CB512601)
