摘要
目的:探讨7种不同胆汁酸对AR42J胰腺腺泡细胞的损伤作用,检测在各种胆汁酸作用下胰腺腺泡细胞的存活率和凋亡/坏死的改变.方法:以大鼠AR42J胰腺腺泡细胞系为研究对象,应用MTT法检测7种不同胆汁酸对细胞存活率的影响和剂量与时间依赖性,采用光学显微镜和荧光显微镜观察细胞形态学改变与凋亡/坏死的变化,流式细胞术AV/PI双染法检测细胞的凋亡/坏死率.结果:CA,GCA和GDCA在0.1-1.0mmol/L内对AR42J细胞不具有损伤作用;而DCACDCA和LCA分别从0.3mmol/L开始,TDCA从0.4mmol/L开始,呈剂量依赖性对AR42J细胞产生损伤作用.0.8mmol/LCA组细胞凋亡率与坏死率同CON组无明显区别(1.2%vs0.9%;1.0%vs1.0%,均P>0.05);0.4mmol/LDCA组细胞凋亡率和坏死率分别为45.2%和8.9%;0.8mmol/LDCA组细胞凋亡率和坏死率分别为18.6%和45.4%.
AIM：To investigate the cytotoxic effects of seven different bile acids on pancreatic acinar AR42J cells by detecting the survival, necrosis and apoptosis of AR42J cells incubated with different bile acids.METHODS：The effects of different bile acids on the survival of rat AR42J cells were detected by methyl thiazolyl tetrazolium（MTT） assay.Cell morphological changes were observed by microscopy and fluorescence microscopy.Cell apoptosis and necrosis were determined by annexin-V and propidium iodide double staining and flow cytometry.RESULTS：Cholic acid（CA）, glycocholic acid（GCA）, and glycodeoxycholic acid（GDCA） at doses ranging from 0.1 to 1.0 mmol/L had no cytotoxic effects on AR42J cells.Deoxycholic acid（DCA）, chenodeoxycholic acid（CDCA） and lithocholic acid（LCA） at doses ≥ 0.3 mmol/L, and taurodeoxycholic acid（TDCA） at doses ≥ 0.4 mmol/L induced cytotoxic effects on AR42J cells in a dose-dependent manner.No significant differences were noted in the apoptotic rate and necrotic rate between untreated AR42J cells and those treated with 0.8 mmol/L of CA（apoptotic rate：1.2% vs 0.9%;necrotic rate：1.0% vs 1.0%）.The apoptotic rate and necrotic rate of AR42J cells treated with 0.4 mmol/L of DCA were 45.2% and 8.9%, respectively, while those treated with 0.8 mmol/L of DCA were 18.6% and 45.4%, respectively.CONCLUSION：Different bile acids exert different cytotoxic effects on AR42J cells.Some bile acids induce the apoptosis and/or necrosis of AR42J cells in a dose-dependent manner.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第3期284-289,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30973851~~
