摘要
目的观察实验性大鼠脑震荡后c-jun蛋白的表达变化规律,探索脑震荡后法医病理学的诊断指标。方法将55只大鼠随机分为脑震荡组和对照组。用免疫组织化学SABC法观察大鼠脑震荡后脑内c-jun蛋白表达变化的规律。结果对照组大鼠可见c-jun蛋白的弱阳性表达。脑震荡组损伤后15min可在神经元观察到c-jun蛋白的表达,随着损伤后经过时间的延长,表达c-jun蛋白的细胞数及范围逐渐扩大,损伤后3h表达达高峰。结论c-jun蛋白的检测可作为诊断脑震荡和推断脑震荡后经过时间的一项敏感指标。
Objective To observe c-jun protein expression after rat brain concussion and explore the forensic pathologic markers following brain concussion. Methods Fifty-five rats were randomly divided into brain concussion group and control group. The expression of e-jun protein was observed by immunohistochemistry. Results There were weak positive expression of c-jun protein in control group. In brain concussion group, however, some neutrons showed positive expression of c-jun protein at 15 rain after brain concussion, and reach to the peak at 3 h after brain concussion. Conclusion The research results suggest that detection of c-jun protein could be a marker to determine brain concussion and estimate injury time after brain concussion.
出处
《法医学杂志》
CAS
CSCD
2010年第1期15-17,共3页
Journal of Forensic Medicine
