摘要
目的:探讨涎腺肿瘤组织中抑癌基因PTEN的突变情况及其与涎腺肿瘤发生、发展的关系。方法:对86份涎腺肿瘤组织应用PCR-单链构象多态性分析技术,检测其中的PTEN基因第5、8外显子的点突变情况。结果:86份涎腺肿瘤组织的PTEN基因第5、8外显子全长序列PCR扩增均获成功,其第5、8外显子均未发现有突变。结论:涎腺肿瘤的发生可能与PTEN基因第5、8外显子的突变无关。
Objective： To investigate the relationship between mutation of tumor suppressor gene PTEN and the development of salivary gland tumor. Methods： Polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） was introduced to detect the mutations of exons 5 and exons 8 of PTEN gene in 86 cases of salivary gland tumor tissues. Results： The whole sequences of exons 5 and exons 8 of PTEN gene in 18 cases of plemorphic adenoma, 30 cases of adenoid cystic carcinoma, 38 cases of mucoepidermoid carcinoma and 20 cases of normal sal- ivary gland tissue specimens were expanded successfully. There was no mutation in exons 5 or exons 8 of PTEN gene in any cases. Conclusion ： There may be no correlation between mutations in exon 5 and exon 8 of PTEN gene and the development of salivary gland tumor.
出处
《新医学》
2010年第1期13-15,共3页
Journal of New Medicine
基金
四川省教育厅自然科学基金重点项目(2004A061)
关键词
涎腺肿瘤
基因
PTEN
单链构象多态性分析技术
突变
Salivary gland neoplasms
Gene
PTEN
Single strand conformation polymorphism
Mutation
