摘要
目的探讨let-7a2在肺腺癌中的表达状态及其意义。方法采用实时定量PCR技术，以U6为内参，对15例手术切除的肺腺癌组织和正常组织进行let-7a2基因表达的检测，检测的数据采用2-△△Cr法进行分析。结果采用配对t检验进行统计学处理。结果在15例患者中，let-7a2在肺腺癌组织中的表达为0．8733±0．4821，较正常肺组织（2．4527±1．0111）明显下调，差异有统计学意义（t=6．816，P〈0．001）。结论let-7a2在肺癌组织中的表达明显下调，可能参与了肺癌发生、发展过程。
Objective To explore the expression and significance of let-7a2 in lung adenocarcinoma. Methods To adapt technology of reahime PCR with reference of U6, the expression of let-7a2 in tumor tissues and normal tissues was detected in 15 cases of resected lung adenocarcinoma. The laboratorial data were analysed by measure of 2-△△ Cr method. The analysed data were managed by statistics. Results The expression of let-7a2 in lung adenoearcinoma tissue was significantly lower than that in lung normal tissue （0.8733 ± 0.4821 vs 2.4527 ± 1.0111）. This difference possess markedness significanee（t = 6.816,P 〈 0.001）. Conclusion The expression of let-7a2 in lung adenocarcinoma is obviously lower and is concerned with the course of lung cancer evolution.
出处
《肿瘤研究与临床》
CAS
2010年第1期49-51,共3页
Cancer Research and Clinic
基金
基金项目：上海市第六人民医院医疗集团科学研究基金（2006-09）
