摘要
目的:建立HPLC法同时测定苍术中苍术素、白术内酯Ⅱ两组分含量。方法:采用Phenomenex C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-水为流动相,梯度洗脱(0～9 min,乙腈-水比例60∶40;9～10 min,乙腈-水比例由60∶40→72∶28;10～20 min,乙腈-水比例72∶28),流速1.0 mL.min-1,检测波长276 nm,柱温24℃。结果:苍术素、白术内酯Ⅱ两组分的检测范围分别为0.00625～0.1875 mg.mL-1(r=0.9997)和0.0044～0.176 mg.mL-1(r=0.9994),平均回收率(n=9)分别为98.3%和97.8%。结论:本法操作简单,结果准确,重现性好,为全面评价不同产地苍术的质量提供了可靠的分析方法。
Objective：To develop an HPLC method for atractylodin and atractylenolide Ⅱ in Rhizoma Atractylodis.Methods：Phenomenex C18（250 mm×4.6 mm,5 μm） column was adopted to separate the sample at the column temperature of 24 ℃.The mobile phase consisted of acetonitrile-water with a gradient elution（0-9 min,60∶40;9-10 min,60∶40→72∶28;10-20 min,72∶28） at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 276 nm.Results：The calibration curve was linear in the ranges of 0.00625-0.1875 mg·mL-1 for atractylodin（r=0.9997） and 0.0044-0.176 mg·mL-1 for atractylenolide Ⅱ（r=0.9994）;The average recoveries（n=9） of atractylodin and atractylenolide Ⅱ were 98.3% and 97.8%,respectively.Conclusion：This method is simple and reproducible,which can be used for the quality control of Rhizoma Atractylodis.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第1期17-20,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
高效液相色谱
苍术素
白术内酯Ⅱ
苍术
HPLC
atractylodin
atractylenolide Ⅱ
Rhizoma Atraetylodis
