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一株高效耐冷菌的分离鉴定及降解特性研究 被引量:13

Separation and identification of an efficient psychrotrophic bacteria and research on its degradation characteristics
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摘要 寒冷地区水温偏低,污水生物处理效果差,出水达标困难。从寒冷地区水土样品中,分离到数株能在2℃下生长的低温菌株,选择优势菌N进行重点研究。从脱氢酶活性机制上,考察了不同条件对N菌株降解能力的影响;对比研究了它与常温活性污泥降解能力和脱氢酶活性的差异。结果表明:N菌株属于耐冷型低温微生物,结合16S rDNA序列同源性分析及生理生化特性鉴定为黄假单胞菌;5℃曝气8 h,接种废水COD、氨氮去除率均在90%以上;低温条件下,该耐冷菌对生活污水降解率比常温活性污泥高出40%,脱氢酶具有更高的活性,在低温污水处理方面具有广阔的应用前景。 Biotreatment techniques,in cold regions,have low efficiency and great difficulty meeting standards.In this paper,several psychrotrophs were obtained from water and soil samples.Strain N,with top high-rate growth,was selected to research.Based on mechanism of dehydrogenase activity,its removal efficiency was analyzed under different conditions.Furthermore,it was compared with activated sludge in dehydrogenase activity and degradation ability.Results indicated that this strain is psychrotrophic.According to homology analysis of 16S rDNA gene sequence and physiological-biochemical characteristics,it belongs to the genus Pseudomonas flava.At 5℃,with aeration time for 8 h,the removal rates of COD、NH3-N can both reach more than 90%.Compared with activated sludge,it has higher dehydrogenase activity and stronger degradation ability(about 40%) at low temperatures.It may have broad application prospect in treatment of cryogenic wastewater.
出处 《环境工程学报》 CAS CSCD 北大核心 2010年第2期377-382,共6页 Chinese Journal of Environmental Engineering
关键词 低温 耐冷菌 生物降解 脱氢酶活性 low temperature psychrotrophs bio-treatment dehydrogenase activity 4
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