摘要
目的了解常州地区单采献血人群中的抗丙型肝炎病毒(HCV)以及输血传播病毒(TTV)-DNA感染的状况,探讨TTV-DNA对单采血小板献血者对丙氨酸转氨酶(ALT)的影响。方法应用酶联免疫吸附试验(ELISA)法检测单采献血小板以及单一献全血人群中的抗-HCV;应用荧光定量聚合酶链反应(FQ-PCR)检测单采血小板献血者以及献全血者TTV-DNA;并应用全自动生化速率法检测TTV-DNA阳性及阴性献血者血清ALT。结果单采献血者抗-HCV、TTV-DNA的阳性率分别为0.15%、14.16%,普通献全血者则分别为0.43%、5.83%;和单一献全血者相比,单采血小板献血者抗-HCV阳性率低于献全血者(χ2=10.22,P<0.01);TTV-DNA阳性率则高于献全血者(χ2=6.57,P<0.05);单采献血者中TTV-DNA阳性和阴性献血者ALT水平分别为(22±7)、(17±10)U/L,两者比较差异无统计学意义(t=0.63,P>0.05)。结论单采献血小板者感染者抗-HCV低于单一献全血者,可能与其反复多次的检验有关;而单采献血者TTV-DNA感染者高于献全血者,可能和其频繁采血有关;单采献血者TTV-DNA阳性对单采献血者ALT无影响,其机制有待进一步的研究。
Objective To investigate the rate of anti-HCV and the transfusion transmitted virus DNA （TFVDNA） in platelet donors in Changzhou area, and to explore the relationship between TFV-DNA and ALT. Methods The anti-HCV antibody was measured by ELISA assay,and TFV-DNA was detected by FQ-PCR, and ALT was measured by auto biochemical method. Results The rate of anti-HCV antibody in platelet apheresis donors was 0.15%,it was lower than that of whole blood donors 0.43% （X^2=10.22, P〈0.01 ）. While the rate of TTV-DNA was 14.16%, and it was higher than that of whole blood donors 5.83% （X^2=6.57, P〈0.05）. There was no significant difference for ALT in serum between TFV-DNA positive platelet apheresis donors [ （22±7） U/L] and TTV-DNA negative donors [ （17±10） U/L] （t=0.63, P〉 0.05）. Conclusion The rate of anti-HCV antibody in platelet apheresis donors was lower than that of whole blood donors, it was due to examining and reexamining. And the rate of TTV-DNA was higher than that of whole blood donors; there was no relationship between TTV-DNA and ALT level, but the underlying mechanisms are unknown.
出处
《实用医技杂志》
2010年第2期112-113,共2页
Journal of Practical Medical Techniques
基金
江苏省常州市卫生局资助(WJ200447)
关键词
血小板去除法
肝炎抗体
丙型
输血传播病毒
丙氨酸转氨酶
Plateletpheresis
Hepatitis C antibodies
Transfusion-transmitted virus
Alanine transaminase
