摘要
目的探讨三种不同训练方式对大鼠骨骼肌IGF-Ⅰ、MGF基因表达的影响。方法32只SD大鼠随机分为安静组(C),长期训练组(T),负重训练组(O)和一次急性负重运动组(AO),每组8只。T组进行8周每天60 min的游泳运动;O组进行4周每天60 min的负重游泳运动;AO组在处死当天负重游泳60 min。T组和O组末次训练后24 h切取腓肠肌,Real-time PCR检测IGF-Ⅰ Ea和MGF的mRNA表达。结果(1)T组IGF-Ⅰ Ea mRNA表达显著高于C组(P<0.05),O组IGF-Ⅰ Ea mRNA表达显著高于C组(P<0.05),AO组IGF-Ⅰ Ea mRNA表达非常显著地高于C组(P<0.01);(2)T组MGF mRNA表达显著高于C组(P<0.05),O组MGF mRNA表达显著高于C组(P<0.05),AO组MGF mRNA表达极显著地高于C组(P<0.01)。结论负重游泳运动致使IGF-Ⅰ Ea mRNA和MGF mRNA表达升高,骨骼肌产生损伤,同时卫星细胞增殖,骨骼肌细胞进行损伤再修复过程。说明IGF-Ⅰ有刺激卫星细胞增殖,并促进增殖的细胞分化,改善骨骼肌结构,修复损伤肌肉。
Objective To investigate the effects of exercise on the gene expression of IGF- I and MGF. Methods 32 male SD rats were randomly and equally divided into four groups： control group （C）, training group （T）, overload training group （O） and acute bout overload exercise group （AO）. The rats of training group take 8 weeks （60 min/d） swimming exercise; the rats of overload training group take 4 weeks （60 min/d） swimming exercise with overload; the rots of acute bout overload exercise group take 60rain swimming exercise with overload before killed. 24h after the last training, the rats of T group and O group were killed, gastrocnernius was excised quickly for Real - time PCR analysis. Results （ 1 ） The IGF - I Ea mRNA expression was upregulated significantly in T and O （ P 〈 0.05）, and was uprcgulated significantly in AO （ P 〈0.01）; （2） The MGF mRNA expression was upregulated significantly in T and O （ P 〈 0.05）, and was upregulated significantly in AO （ P 〈 0.01 ）. Conclusion The expression of skeletal muscle IGF - I Ea and MGF mRNA in overload group and acute bout overload group significantly increased compared with control group, indicated that muscle satellite cell were stimulated and were required for local muscle repair by IGF- I.
出处
《西安体育学院学报》
CSSCI
北大核心
2010年第1期80-83,共4页
Journal of Xi'an Physical Education University
