摘要
目的研究肝硬化大鼠模型肠黏膜结构的改变及细菌移位。方法采用皮下注射40%四氯化碳诱导大鼠肝硬化模型,比较肝硬化大鼠模型与正常对照组小肠组织结构及超微结构的变化;将肝、脾和肠系膜淋巴结组织匀浆进行细菌培养,了解肠道细菌移位情况;采用鲎实验检测血浆内毒素水平。结果与正常对照组相比,肝硬化模型组小肠绒毛表面积显著下降(P<0.01),而肠隐窝深度无明显变化(P>0.05),肠黏膜上皮细胞微绒毛明显缩短、稀疏,上皮细胞间紧密连接结构模糊,细胞间隙增大;肝硬化模型组肝、脾、肠系膜淋巴结细菌移位发生率分别为16.67%、16.67%和41.67%,正常对照组未发现细菌生长;肝硬化模型组血浆内毒素水平明显高于正常对照组(P<0.01)。结论肝硬化大鼠模型肠黏膜上皮组织及超微结构发生改变,从而导致肠道细菌移位和血浆内毒素水平的升高。
Objective To investigate the morphology of intestinal mucosa and bacterial translocation in the rat of cirrhosis.Methods The rat model of cirrhosis was made by administering 40% carbon tetrachloride.The mucosal structure was observed under light and electronic microscopes.The homogenates of the liver,spleen and mesenteric lymph node from two experimental groups were cultured separately on blood agar plates.The endotoxin was measured by limulus endotoxin analysis kit.Results The villus height and surface area in the cirrhotic group significantly decreased（P0.01）,but the crypt depth had no difference between the cirrhotic group and the control group（P0.05）.The microvillus of epithelial cell was short,and the tight junction was vague in cirrhotic group.The incidence rates of bacterial translocation to liver,spleen and mesenteric lymph node were 16.67%,16.67% and 41.67% respectively in cirrhotic group,but no bacterial translocation found in the control group.The level of endotoxin in the cirrhotic group was higher than that in the control group（P0.01）.Conclusion The pathology and ultrastructure change in the cirrhotic group,and this induces the bacterial translocation and endotoxemia.
出处
《胃肠病学和肝病学杂志》
CAS
2010年第1期25-27,共3页
Chinese Journal of Gastroenterology and Hepatology
关键词
肝硬化
超微结构
细菌移位
内毒素
Cirrhosis
Ultrastructure
Bacterial translocation
Endotoxin
