摘要
目的探讨重组人抵抗素对甲状腺相关眼病(TAO)患者眼眶前脂肪细胞分化的影响及其在TAO发病中的可能作用。方法10例严重TAO患者眼眶脂肪组织来自眼眶减压术中,正常对照组来自各种原因行眼球摘除术者。培养眼眶前脂肪细胞,并诱导分化,在分化液中分别加入0、10、25、50、100μg/L质量浓度的重组人抵抗素,采用逆转录PCR(RT-PCR)法检测分化过程中过氧化物酶增生体激活受体γ(PPARγ)基因的表达,研究重组人抵抗素对前脂肪细胞分化的作用。结果TAO患者及正常对照组眼眶前脂肪细胞在体外均可被诱导分化成脂肪细胞。分化成熟的脂肪细胞可被油红O染成橘红色。重组人抵抗素对眼眶前脂肪细胞的分化有抑制作用,RT-PCR结果显示PPARγ基因的表达随着抵抗素质量浓度的升高而减弱,差异有统计学意义(P<0.05)。结论重组人抵抗素可抑制人眼眶脂肪组织来源前脂肪细胞的分化,脂肪细胞标记性PPARγ基因的表达减弱,有望在TAO治疗方面开创新的研究方向。
Background The enhance of differentiation of orbital preadipoeyte is a main factor for pathogenesis of thyroid-associated ophthalmopathy （TAO）. Experiment has proved that orbital preadipocyte can differentiate into mature adipoeyte expressing resistin,and resistin is a adipocyte factor with biological activity. Objective The present study is to investigate the effects of recombinant human resistin on differentiation of orbital preadipocytes derived from TAO patients orbital tissues. Methods Orbital adipose tissue was obtained from 10 eyes with TAO during the orbital decompression surgery. Orbital adipose tissue of the normal group was obtained from 5 removed eyes during the surgery due to different reasons. Preadipocytes were isolated and cultured using expand culture method. The 2 - 5 generations of preadipoeytes were identified by oil red O staining under the light microscope. 0,10,25,50,100 ng/mL of recombinant human resistin were added into medium in the first day after differentiation of cells. The expression of PPAR3, mRNA in differentiated adipocytes were detected by reverse traneripaton PCR（RT-PCR）. Results The differentiation of preadipocyte presented the similar procedure and outcome between the TAO group and normal group. Differentiated adipocyte was stained into the jacinth by oil red O. The expression of PPARγ mRNA in differentiated cells was gradually declined with the increase of concentration of recombinant human resistin （F = 241. 341 ,P 〈 0.01 ）. A dose-dependent inhibitory effect was detected in the expression of PPARγ mRNA. The weakest expression of PPARγ mRNA in differentiated cells was 100 ng/mL resistin group. Conclusion The recombinant human resistin has an inhibitory effect on the differentiation of orbital preadipocytes by reducing the expression of adipose-related genes. Resistin is probably a new research approach to treatment of TAO.
出处
《眼科研究》
CSCD
北大核心
2010年第2期167-170,共4页
Chinese Ophthalmic Research
基金
"211工程"重点学科建设基金资助(98005)
关键词
甲状腺相关眼病
前脂肪细胞
抵抗素
thyroid-associated ophthalmopathy
preadioeyte
resistin
