摘要
目的观察骨髓间充质干细胞(MSCs)对电离辐射诱发的小鼠胸腺瘤的抑制作用。方法采用经典Kaplan法复制电离辐射诱发的小鼠胸腺瘤模型。应用全骨髓贴壁法分离培养C57BL/6小鼠MSCs,DAPI标记,经尾静脉注入荷瘤小鼠后,分别于1、5、10d处死小鼠,取胸腺组织,激光共聚焦显微镜下观察MSCs在胸腺瘤组织中的定位;第1次全身大剂量照射后6个月取胸腺组织,HE染色观察胸腺组织的病理变化,并判断成瘤情况。结果激光共聚焦显微镜下观察可见,MSCs经尾静脉输注后可迁徙至小鼠胸腺组织内;病理观察显示,胸腺组织皮髓质结构清楚,淋巴样肿瘤细胞较少,细胞形态、大小不一,偶见核分裂象;MSCs输注使辐射诱导的胸腺瘤成瘤率由57.00%±9.78%降低至37.50%±7.55%。结论已成功建立辐射诱发的小鼠胸腺瘤模型;输注的MSCs可迁徙至胸腺组织中,并降低胸腺瘤的成瘤率。
Objective To observe the inhibitory effect of mesenchymal stem cells(MSCs)on the thymoma induced by ionizing radiation in mice.Methods The mouse model of thymoma was replicated by ionizing radiation by typical Kaplan method.The MSCs of C57BL /6 mice were isolated and cultured by whole bone marrow adherent culture method,labeled with DAPI and injected into model mice through tail vein.Partial model mice were killed 1,5 and 10 d after injection respectively,and their thymus tissues were observed for location of MSCs in thymoma by laser confocal microscopy.The thymus tissues of partial model mice were collected 6 months after the first high dose systemic radiation,and observed for pathological change by HE staining,based on which the thymoma formation was judged.Results Laser confocal microscopy showed that the MSCs injected through tail vein were migrated to the thymus tissues of mice.Pathological observation showed that the structures of cortices and medulla of thymus tissues of mice treated with MSCs were clear,while lymphoid tumor cells were in a small number and uneven shape and size,and nuclear division was observed occasionally.The thymoma formation rate of mice treated with MSCs was 37.50% ± 7.55%,which decreased significantly as compared with that of model mice untreated(57.00% ± 9.78%).Conclusion The mouse model of thymoma induced by ionizing radiation was successfully established.The MSCs injected may be migrated to the thymus tissue and decrease the thymoma formation rate.
出处
《中国生物制品学杂志》
CAS
CSCD
2010年第1期36-38,50,共4页
Chinese Journal of Biologicals
基金
吉林省科技厅科研基金资助课题(20080446)
关键词
骨髓间充质干细胞
电离辐射
胸腺瘤
Mesenchymal stem cells (MSCs)
Ionizing radiation
Thymoma