摘要
目的观察小鼠骨髓间充质干细胞(Mesenchymal stem cells,MSCs)移植后,在异基因肝移植模型大鼠胸腺、脾脏及骨髓中的迁徙、定居情况及对淋巴细胞增殖的抑制作用,以探讨MSCs诱导异基因移植免疫耐受的可能性。方法体外分离、纯化并培养小鼠MSCs;将小鼠肝组织块埋入大鼠肝脏切口,建立异基因肝移植大鼠模型;经尾静脉移植DAPI标记的小鼠MSCs,通过激光共聚焦显微镜观察24 h及5 d时,MSCs在模型大鼠胸腺、脾脏及骨髓内的迁徙及定居;采用体外淋巴细胞增殖实验检测小鼠MSCs对异基因肝移植大鼠淋巴细胞增殖的抑制作用。结果MSCs移植后,24 h即散在分布于胸腺、脾脏及骨髓内,5 d时集中在血管周围;经MSCs治疗后,大鼠胸腺和脾脏淋巴细胞增殖均受到抑制,与未经治疗的模型大鼠相比,差异有显著意义。结论小鼠MSCs可迁徙至异基因肝移植大鼠免疫器官内,并具有抑制淋巴细胞增殖的作用。
Objective To observe the migration and colonization of murine bone marrow-derived mesenehymal stem cells （MSCs） in the thymus, spleen and bone marrow of model rats with xenogenic liver transplant as well as the inhibitory effect of MSCs on lymphocyte proliferation, and explore the possibility of induction of immunotolerance to xenogenic liver transplant by MSCs. Methods Establish rat model of xenogenic liver transplant by imbedding the hepatic tissue pieces of KM mice into the cut of liver of Wister rats. The MSCs of suckling KM mice were isolated, purified and cultured in vitro, then labeled with DAPI and injected into the caudal vein of model rats. Observe the migration and colonization of MSCs in thymus, spleen and bone marrow by laser scanning confocal microscopy 24 h and 5 d after injection. Determine the inhibitory effect of MSCs on the proliferation of lymphocytes of model rats by lymphocyte proliferation test in vitro. Results MSCs were observed in thymus, spleen and bone marrow 24 h and distributed around blood vessels 5 d after injection. Both the proliferations of lymphocytes in thymus and spleen of model rats treated with MSCs were significantly inhibited as compared with those untreated with MSCs. Conclusion Murine MSCs were migrated to the immune organs of rats with xenogenic hver transplantation and showed inhibitory effect on lymphocyte proliferation.
出处
《中国生物制品学杂志》
CAS
CSCD
2008年第4期312-314,322,共4页
Chinese Journal of Biologicals
基金
吉林省卫生厅医学科研基金项目(200313)
关键词
骨髓间充质干细胞
肝脏
异基因移植
迁徙
定居
淋巴细胞增殖
抑制
Bone marrow-derived mesenchymal stem cells
Liver
Xenogenie transplant
Migration
Colonization
Lymphocyte proliferation
Inhibition
