摘要
目的探讨不同剂量亚砷酸钠对体外培养人淋巴细胞金属硫蛋白（MT）基因表达的影响及MT表达与细胞存活率的相互关系。方法无菌抽取健康人血液，分离出淋巴细胞，分别用0（对照）、2、5、10、15、30、60μmol／L亚砷酸钠作用于淋巴细胞，于24、48、72h后，以四噻唑蓝比色法测定细胞存活率；染毒72h后采用RT—PCR法检测MT-1、MT-2基因表达情况。结果2、5μmoL／L染毒组细胞存活率[（115．50±11．80）％、（130．49±8．28）％]高于对照组[（100．00±0．00）％，P均〈0．05]；10、15、30、60μmol／L染毒组细胞存活率『（78．12±9．33）％、（71．62±10．82）％、（52．06±3．05）％、（40．98±5．41）％]随染毒剂量增高而逐渐降低，与对照组及2、5μmol／L组比较差异有统计学意义（P均〈0．05）。不同剂量亚砷酸钠作用72h，对照组及2、5、10、15、30、60μmol／L染毒组MT—1基因表达（0．925±0．123、1．082±0．504、1．103±0．170、0．9274-0．056、0．730±0．307、0．604±0．173、0．540±0．075）组间比较差异无统计学意义（P均〉0．05）；2、5μmol／L染毒组（1．503±0．212、1．557±0．377）与对照组（0．762±0．112）比较MT-2表达上调（P均〈0．05），且较其他各组表达增加（P均〈0．05），而10、15、30、60μmol／L染毒组MT-2表达（0．814±0．139、0．679．4-0．201、0．607±0．229、0．533±0．102）呈下降趋势，但组间比较差异无统计学意义（P均〉0．05）。MT-1、MT-2表达水平与细胞存活率之间存在正相关关系（r值分别为0．955、0．909，P均〈0．05）。结论10μmol／L以上剂量的砷可能对淋巴细胞MT表达有抑制作用，而低剂量（2、5μmol／L）砷可能刺激淋巴细胞的MT-2表达上调，提高细胞MT水平，起到保护细胞、提高细胞存活率的作用。
Objective To explore the effects of sodium arsenic on Metallothionein（MT） isoforms genes expression, and to study the relevance between the MT expression and cell survival percentage. Methods Healthy persons blood was extracted aseptically and the lymphocytes were separated. The lymphocytes were treated by 0 （control）,2,5,10,15,30,60 μmol/L sodium arsenites, respectively. The cell survival percentage was determined by methyl thiazolyl tetrazolium（MTr） reduction assay at 24,48,72 h intervals, while the expression of MT-1 and MT-2 were examined by RT-PCR in 72 h. Results The cell survival percentage in 2,5 μmol/L groups were （115.50 ± 11.80）% and （130.49 ± 8.28）%, which were all higher than those in the control group[（100.00 ± 0.00）%, all P 〈 0.05] at any intervals. In 10, 15,30,60 μmol/L groups, the cell survival percentage[（78.12 ± 9.33）%, （71.62 ± 10.82） %, （52.06 ± 3.05） %, （40.98 ± 5.41 ） % ] increased along with the decrease of concentration, which showed a significant difference between these groups and the control group and 2,5 μmol/L groups（all P 〈 0.05）. There was no significant difference on MT-1 expression after exposed to different concentration sodium arsenites （0,2,5,10, 15,30,60 μmol/L） for 72 h（0.925 ± 0.123,1.082 ± 0.504,1.103 ± 0.170,0.927 ± 0.056,0.730 ± 0.307,0.604 ± 0.173,0.540 ± 0.075, all P 〉 0.05）. The expression of MT-2 in 2,5 μmol/L groups（1.503 ± 0.212,1.557 ± 0.377）was up regulated compared with that in control group（0.762 ± 0.112） and MT-2 also expressed more than that in other groups（all P 〈 0.05）. However, the expression of MT-2 declined when the concentraion was 10,15,30, 60 μmol/L（0.814 ± 0.139,0.679 ± 0.201,0.607 ± 0.229,0.533 ± 0.102）. There was no significant difference among the groups（all P 〉 0.05 ）. The expression level of MT-1, MT-2 was positively correlated with the cell survival percentage （r = 0.955,0.909, all P 〈 0.05）. Conclusions The sodium arsenite at co
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2010年第1期9-12,共4页
Chinese Jouranl of Endemiology
基金
基金项目：国家自然科学基金（30560137）
关键词
砷
金属硫蛋白
基因表达
淋巴细胞
Arsenic
Metallothionein
Gene expression
Lymphocytes
