摘要
银鲫(Carassius auratus gibelio)是行雌核发育的三倍体物种,但在自然水域与二倍体普通鲫(Carassius auratus L.)生活在一起,外形上无法区分,在群体遗传学的各种参数上两者也没有明显区别。本研究利用17个二碱基微卫星标记和51个三碱基微卫星标记对2次采样共6个样本组进行了基因型分析。通过对电泳图案及电泳结果的简单描述和比较来分析银鲫和普通鲫在群体遗传学上的差别。在2次采样所得群体的等位基因数和基因型数这2个参数上,普通鲫都明显高于银鲫(第1次所采样本普通鲫与银鲫的基因型数之比217∶175;等位基因数之比188∶163;第2次样本基因型数之比537∶349,等位基因数之比353∶282)。结果也表明在纯合子占优势的基因座,纯合子比例银鲫高于普通鲫,杂合子比例则是普通鲫远远高于银鲫;与此完全相反,在杂合子占优势的基因座,纯合子比例银鲫低于普通鲫,杂合子比例则是银鲫远远高于普通鲫。研究还通过比较普通鲫的基因座遗传平衡数据,讨论了银鲫群体基因座不平衡现象的特征和产生的原因。
Silver crucian carp （Carassius auratus gibelio） is a gynogenetic species and lives in the same body of water with normal diploid crucian carp （C. auratus L.）. The two kinds of fish can not be discriminated through phenotype, while there is no obvious difference in genetic parameters between the two populations. In this study, electrophoresis patterns of the PCR products for the two crucian carps and the simple description of these patterns were used to find out the difference between silver crucian carp and normal diploid crncian carp in population genetic composition. 17 SSR markers with two motifs of repeated sequences and 51 SSR markers with three motifs of repeated sequences were used to analyze the genotypes of 6 sample groups obtained in two times. The number of allele is much more in normal crncian carp than silver crucian carp （For the first time samples, the ratio of genotypes is 217 ： 175, and the ratio of alleles is 188 ： 163; for the second time samples, the ratio of genotypes is 537 ： 349, and the ratio of alleles is 353 ： 282 ）. There are distinct difference between crucian carp and silver crucian carp on the patterns of electropgoresis pictures of PCR products. The results also showed that in the locus where most individuals are homozygotes, silver crucian carp have more homozygotes and much fewer heterozygotes than normal crncian carp. In contrast, silver crucian carp have more heterozygotes and fewer homozygotes than crncian carp in the locus where most individuals are heterozygotes. In the paper, by comparing the data of genetic equation between crucian carp and silver crucian carp, imbalance of silver crucain carp in each locus and its origin were discussed. Our data suggested that the two crucian carps are very different in population genetic composition.
出处
《中国水产科学》
CAS
CSCD
北大核心
2010年第1期1-10,共10页
Journal of Fishery Sciences of China
基金
国家自然科学基金项目(30271010)
关键词
野生群体
银鲫
雌核发育
普通鲫
wild population
Carctssius auratus gibelio
gynogenesis
Carassius auratus L.
