摘要
本文探讨用分光光度法测定血红素中铁离子的含量。通过对血红素进行硝化处理，将其中铁离子游离出，并与适量的邻菲罗啉在pH为4.6的缓冲液中定量生成橙红色络合物，用分光光度法在510±1nm处测定其含量。结果显示，铁离子与邻菲罗林生成的橙红色络合物在510±1nm波长处有最大吸收，且在0．4～2．8μg／ml范围内，浓度与吸收度符合Beer定律，相关系数r＝0．9999，方法平均回收率X＝98．87％，RSD＝0．58％。本法结果准确，方法可行，适用于医院、药厂生产的各种血红素制剂的质量分析与检验，并可为医生用药提供依据。
Iron-ion in Hematin was determination by spectrophotometry at wavelengths of 510± 1nm.The result showed a good linear relationship in the concentration range of 0. 4~ 2, 8 μg/ml (r =0. 9998).The average recovery rate was 98. 87 %,the coefficient of variation was 0. 58 %. The method is feasible,the result is accurate. It is suitable for analysis of hospital preparation and pharmaceutical factory.
出处
《海峡药学》
1998年第2期34-35,共2页
Strait Pharmaceutical Journal
