摘要
目的:设计并表达预防16型人乳头瘤病毒感染的重组蛋白疫苗。方法:选取HPV16型病毒L1蛋白上的B表位和T表位,将其连接组合成多种方式,利用计算机预测蛋白质的三维结构,选取其中最优的结构进行基因克隆构建,并利用杆状昆虫系统进行表达。结果:利用计算机设计出了一种重组蛋白,成功构建了该重组蛋白的结构基因,并在杆状昆虫表达系统中得到表达。结论:成功构建并表达了人乳头瘤病毒的重组蛋白疫苗。
AIM：To develop a recombinant protein vaccine with epitopes on L1 protein for prevention of HPV16 infection.METHODS：One B cell epitope and one T cell epitope from HPV16 L1 protein were chosen to construct genes encoding different recombinant proteins.Then all the 3D structures of the proteins were predicted by Geno3D in internet.An optimal protein named RH was chosen in which three B epitopes＇ location simulates that in the real HPV16 L1.Gene X（consisting of B＋T＋T）and gene Y（consisting of B＋T）were constructed by multiple PCR.RH gene（consisting of X＋X＋X＋X＋Y＋Y＋Y）was constructed,sub-cloned into pFastbac1,and expressed in Sf9.RESULTS：RH protein with epitopes on HPV16 L1 was designed and predicted by Geno3D.RH gene was constructed and sub cloned into pFastbac1.Finally,it was successfully expressed in baculovirus expression system.CONCLUSION：A HPV16 recombinant vaccine is successfully constructed and expressed.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2010年第1期38-40,共3页
Chinese Journal of Cellular and Molecular Immunology
