摘要
在脊髓灰质炎（脊灰）病毒分离鉴定中，细胞的选择非常重要，以往世界卫生组织（ＷＨＯ）推荐使用Ｈｅｐ－２和ＲＤ两种细胞同时进行脊灰病毒的分离鉴定。由于这两种细胞不仅对脊灰病毒敏感，对非脊灰肠道病毒（ＮＰＥＶ）同样敏感，分离到许多ＮＰＥＶ。最近ＷＨＯ推荐了一种新型的对脊灰病毒特异的Ｌ２０Ｂ细胞，我们使用这种细胞进行的实验显示。
For the aim of eradicating poliomyelitis, Hep-2 and RD celllines recommended by WHO have been used for poliovirus isolation and identification, but they are not only sensitive to poliovirus but also to non-polio enteroviruses, so that most of the work on sample testing proceeded in the latoratory are not specifically targeted at poliovirus. If cells only specifically sensitive to poliovirus can be recommended, the poliovirus isolation will be more convenient. Recently the poliovirus-specific L20B cells which is a cell line of transgenic mouse barbouring poliovirus receptors are recommended by WHO to the polio laboratory net of China. We used the L20B cells to isolate poliovirus from fecal samples and demonstrates the sensitivity and specificity of L20B cells to the polioviruses.
出处
《中国计划免疫》
1998年第5期268-269,共2页
Chinese Journal of Vaccines and Immunization
